...tral to all T cell responses is the interaction between the T cell receptor and peptide antigen presented by the MHC molecules. This interaction selects the dominant T cell clones with the most favourable (not necessarily highest) affinity and determines the extent of cross-reaction with epitope var...

...tic DC in vivo. DC present peripheral Ags to T cells in lymph nodes, but also influence their differentiation (tolerance/immunity, Th1/Th2). To investigate how peripheral conditions affect DC properties and might subsequently regulate T cell differentiation, we examined the effects of a potent DC-a...

...at bind strongly to L-SIGN, but to a lesser degree or not at all to dendritic cell-specific ICAM-grabbing nonintegrin (DC-SIGN). As a first step to consider the potential of these Abs for Ab-mediated Ag delivery, we evaluated specific peptide delivery to human dendritic cells. A durable human T cell...

... regulatory T cells are present in the colon of untreated mice, but absent in axenic animals, suggesting that their development is dependent on the intestinal flora. We will complete our experiments to further test the role of CD70 in Th1 priming in the presence or absence of regulatory T cells, and...

... DC (pDC), or can be directly isolated from the spleen, lymph nodes or tissue as classical DC (CD11cbright, CD8+ or CD8-) or plasmacytoid pDC. We have shown that macrophages, myeloid DC and plasmacytoid DC have the intrinsic capacity to produce different amounts of IL-12 upon stimulation through T...

...obtained on the properties of the CD16, CD1c and BDCA-3 DC populations and their production of cytokines in response to different Toll-like receptor (TLR). We studied two major blood populations of DCs, myeloid DCs (mDCs) and plasmacytoid DCs (pDCs). Among mDCs, three populations could be further i...

...s, and its effect on the DC differentiation/activation pathway induced by type I IFN. 1,25(OH)2D3 prevented the generation of IFN-DCs when added to freshly isolated monocytes, and is capable to redirect already differentiated IFN-DCs toward a more immature stage. Interestingly the suppressive effect...

... that indirect signals alone led only to partial DC activation and resulted in clonal expansion of T cells lacking typical Th1 or Th2 function. These results show that indirect signals by themselves cannot fully substitute for TLR triggering in DC and further suggest that the function of TLRs and ot...

...tothrix FP1 that we named CyP. We found that CyP acts as a potent and selective antagonist of bacterial LPS. CyP did not induce any detectable response in human DCs and competitively inhibited LPS binding to the extracellular domain of TLR4. Addition of CyP together with LPS completely inhibited bot...

...obtained data on the induction of CTL responses in vivo by immunization of in vitro matured DC versus CTL responses induced by sustained delivery of a maturation signal from a comparison in a murine model. PolyI:C12U (Ampligen) did not activate the murine bone-marrow derived DC. Similar experiment...

... or alpha IFN to promote Mart-1 specific T cell priming and/or NK cell triggering in HHD2 mice. We demonstrated that only TLR3 and 9 ligation is able to induce CTL priming in vivo. However, exosomes in the absence of adjuvants can induce NK cell activation. DC exosome-mediated NK cell triggering dep...

...and post-translational modifications involved in the regulation of specific signalling pathways or cell processes. First, we have set up a system for SILAC labelling of bone marrow derived murine dendritic cells (BM-DC). The principal method for generating BM-DC with GM-CSF was adapted from Lutz et...

...wed that the ballistic delivery of micro-particles to the viable epidermis can result in localised cell death. Furthermore, experimental results show the degree of cell death is dependant on the number of micro-particles delivered per unit of tissue surface area. These results suggest that optimizat...

...e of regulatory T cells. Natural regulatory T cells (CD4+ CD25+) were depleted by injection of anti-CD25 mAbs, whereas regulatory T cells were induced by injection of (presumably agonistic) anti-CTLA-4 mAbs. Our observations demonstrate that natural and CTLA-4 induced regulatory T cells are distinct...

...al zone dendritic cells mediate the cholera toxin adjuvant effect. We demonstrated in mice that a series of critical interactions between the cholera toxin adjuvant and the dendritic cells of the splenic marginal zone lead to effective priming of an immune response. Thus, for the first time, we have...

Data was obtained on RNA transfected DC which proved superior in priming naïve T cells in vitro compared to DC loaded with native peptides.

...L-S+ DC compared to E/L-S- DC. To demonstrate in-vivo that multiple LN regions can be targeted by i.v. application of E/L-S DC, we established indium111 labelling of DC and studied its effect on DC migration and T-cell interaction in vitro prior going into patients. In terms of migratory capacit...

...the ability of mMature DCs to efficiently present antigens associated with MHC molecules to T lymphocytes. Dendritic cells (DCs) are major actors of innate and adaptive immune responses. Immature DCs express a great diversity of receptors at their cell surface, which provide them the ability to upt...

...stration that the target of FcgammaRs with the antigen induces the internalization of the IC, leading to the maturation of CD11c+CD11b+ conventional DCs. These DCs become thus efficient to present antigenic peptides to helper CD4 T cells and cytotoxic CD8 T cells via cross-presentation pathway. This...

...viously shown that conventional DCs express the inhibitory type II FcgammaR and the activatory type I and type III FcgammaRs. Therefore, we next investigated the expression and function of these murine FcgammaR in CD11c+CD11b-B220+ plasmacytoid DCs (pDCs). pDCs express mostly FcgammaRIIB while the e...