Monitoring of the capacity of splenic DC to induce immunity

Data was obtained of the capacity of splenic DC, pulsed in vitro with protein antigens or peptides, to induce immunity after transfer into syngeneic animals. To improve existing strategies, the amplitude and polarisation of T cell responses was monitored in the presence or absence of regulatory T cells. Natural regulatory T cells (CD4+ CD25+) were depleted by injection of anti-CD25 mAbs, whereas regulatory T cells were induced by injection of (presumably agonistic) anti-CTLA-4 mAbs. Our observations demonstrate that natural and CTLA-4 induced regulatory T cells are distinct populations. Both cell types downregulate Th1-type responses, suggesting new strategies to optimize DC-based immunotherapy to induce tumor resistance.

• cell type
• T cell,
• dendritic cell,
• T helper cell 1,
• regulatory t cell

• organ type
• human spleen

• molecule type
• peptide,
• Biomaterial,
• CD25 receptor,
• CD4

created over 16 years ago (2 March 2009)    last modified over 12 years ago (9 July 2012)   [ RDF ]   [ RelFinder ]