We have obtained data on the immunogenicity induced by chemotherapeutics.

...itive plasma cells is located in the BM. Staining for these cells revealed that plasma cells are scattered through the BM and are not preferentially found in DC-B cell niche. However, depletion of DC led to reduction in IgA+ plasma cells, which might indicate that bmDC are critical for survival of t...

Data was generated on DC activation by different forms of the non-pathogenic yeast Saccharomyces cerevisiae. A special focus was laid on the response of MDDCs to the non pathogenic fungi.

... and melanoma from this trial using cellular immunotherapy with dendritic cells loaded with tumor antigens. This study is not yet open for participant recruitment. ClinicalTrials.gov Identifier: NCT00610389 Purpose Background: cellular immunotherapy with dendritic cells (DC) loaded with tumor ant...

Transcriptional data is available on macrophage knockout BALB/c and C57BL/6 mice using different stimuli at different time points in the presence or absence of a kinase inhibitors.

...of the direct Treg-CD8+ T cell contacts that we imaged. Besides, Treg inactivation also induces an increase in the number of dendritic cells (DCs) in these tumor-draining lymph nodes. Therefore Tregs could influence CD8+ T cell arrest by modulating the number of antigen presenting cells in the tumor...

...urface sensing of translation) was applied to monitor translation by FACs during DC/T cell interaction in presence or absence of cognate antigenic peptides. It was demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. Surprisingly, thi...

...luation of different stimulation conditions for the cytometric assessment of antigen-specific Th-cells based on CD154 expression induced during short-term in vitro activation, a method established by us during previous DC-THERA activities. This is important and essential for implementation of the te...

...cence microscopy to analyse the endocytic pathway in DC and to track C-type lectins that are internalised from the cell surface. From these analyses, there is data available. As part of this work, we have used microscopy to study the effect of Dectin-1 uptake on its ability to signal for downstream...

Work has continued to characterise the function of a novel tick-derived DC modulator that perturbs various signalling pathways of human DC but not others; transcriptional profiling and pathways-based analysis will be undertaken in the next phase and data will become available.

Concerning the pathway based analysis and database we have started the curation of the TLR4-CD14 pathway in DCs and we have described the events occurring at the DC membrane level. We have also started global gene expression analyses to better difine the role of NFAT in DCs.

...lts show that in mice with CD8+ T-cells primed with a reactive lymph node the viral load in the lungs was significantly smaller then in a control mice. We also determined that suboptimally primed CD8+ T-cells can develop into functional memory cells. Although suboptimally primed CD8+ T-cells do not...

...rt during human monocyte-derived DC maturation. We have published that the E3-ubiquitin ligase MARCH I is responsible for peptide-loaded MHC II ubiquitination and their subsequent internalization in immature DC.s The ligase MARCH I has been shown to be down-regulated upon activation. (De gassart et ...

... data is available from MRI tracking of dendritic cells in melanoma patients for cellular therapy. In vivo magnetic resonance tracking of magnetically labeled cells is feasible in humans for detecting very low numbers of dendritic cells in conjunction with detailed anatomical information. MRI cell t...

Data will be obtained from a study that we plan on DC receptor characteristics and signalling, focussing on antigen receptors.

...rtments that are formed upon cell entry of antigens via specific pathways. The ultimate goal is to get a better insight into the protein complexes that are involved in antigen processing, and learn whether the different entry routes for antigens into DCs might have distinct functional characteristic...

A large reference ‘golden experiment’ will be carried out in which the transcriptome of DC vaccines of 50-100 patients will be compared and data transcriptomic data will become available.

...ill start a collaboration with G. Schuler (P06) to examine the modulation of dendritic cell function by Denileukin Difitox (ONTAK) and will provide pathway analysis data.

... a receptor for antigen targetting to DC. Notably, tumour antigens coupled to anti-DNGR-1 antibodies and given together with a suitable adjuvant elicit potent CTL responses that can promote tumour regression. We will continue to explore the use of DNGR-1 as a tool for targeting antigens to DC in v...

We have used agonists of the dectin-1/Syk pathway as adjuvants in vivo to induce CD8+ T cell responses and have data available on this. In summary, we have shown that this can result in CTL capable of destroying tumours.