Monitoring translation during DC/T cell interaction

There is data available on translation during DC/T cell interaction in presence or absence of cognate antigenic peptides.

Efforts on the definition of a novel way of monitoring DC and T cell activation as well as studying viral infections were finalized. The puromycin-based technology (SUnSET, surface sensing of translation) was applied to monitor translation by FACs during DC/T cell interaction in presence or absence of cognate antigenic peptides. It was demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. Surprisingly, this phenomenon was mTOR independent (Rapamycin insensitive).

• cell type
• T cell,
• dendritic cell

• molecule type
• peptide

created over 16 years ago (7 December 2009)    last modified over 13 years ago (8 October 2012)   [ RDF ]   [ RelFinder ]