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Monitoring translation during DC/T cell interaction

imaging dataset

There is data available on translation during DC/T cell interaction in presence or absence of cognate antigenic peptides.

Efforts on the definition of a novel way of monitoring DC and T cell activation as well as studying viral infections were finalized. The puromycin-based technology (SUnSET, surface sensing of translation) was applied to monitor translation by FACs during DC/T cell interaction in presence or absence of cognate antigenic peptides. It was demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. Surprisingly, this phenomenon was mTOR independent (Rapamycin insensitive).



created over 8 years ago (7 December 2009)    last modified over 5 years ago (8 October 2012)   [ RDF Rdf ]   [ RelFinder Relfinder ]