Priming of CD8+ T cells: regulation of proliferation and development into effector and/or memory cells

We obtained microarray and RT-PCR data on the priming of CD8+ T cells in mice and their regulation of proliferation and development into effector and/or memory cells.

The positive influence of a reactive lymph node on the priming of CD8+ T-cells was already analyzed in 2007. We set up experiments to determine if this effect would benefit a response against a second infection. We infected mice with CD8+ T-cells primed with or without the influence of a reactive lymph node with a flu-virus encoding the specific antigen. After several days we determined the viral load in the lungs with RT-PCR. The results show that in mice with CD8+ T-cells primed with a reactive lymph node the viral load in the lungs was significantly smaller then in a control mice.
We also determined that suboptimally primed CD8+ T-cells can develop into functional memory cells. Although suboptimally primed CD8+ T-cells do not display good effector function, as previously enclosed, we found that they were efficient at eradicating tumors in the memory phase.
Micro-array data obtained from samples from suboptimally primed CD8+ T-cells compared with optimally primed CD8+ T-cells showed several interesting genes involved in regulation of proliferation and development.

We are currently working on verifying the data obtained with micro-arrays through RT-PCR, and comparing data from in vitro primed CD8+ T-cells to in vivo primed CD8+ T-cells. We intend to analyse the candidate genes through overexpression and siRNA experiments.

• cell type
• T cell

• molecule type
• CD8

• organ type
• human lymph node,
• lung

• organism type
• Mus musculus

• stimulus type
• Viruses

created over 15 years ago (8 December 2009)    last modified over 12 years ago (5 November 2012)   [ RDF ]   [ RelFinder ]