Identification of specific markers: CD16, CD1c and BDCA-3 in mDCs and pDCs

Data was obtained on the properties of the CD16, CD1c and BDCA-3 DC populations and their production of cytokines in response to different Toll-like receptor (TLR).

We studied two major blood populations of DCs, myeloid DCs (mDCs) and plasmacytoid DCs (pDCs). Among mDCs, three populations could be further identified by specific markers: CD16, CD1c and BDCA-3. The properties of these DC populations and their production of cytokines in response to different Toll-like receptor (TLR) agonists were evaluated. The TLR agonists used were: PAM3CSK4, Poly I:C, LPS, Flagellin, Imiquimod, Resiquimod, CpG 2216, CpG 2006. While BDCA-3+ cells seem not have any capability to respond to TLR agonists, CD16 and CD1c produce a number of cytokines in response to these stimuli, with the exception of CpGs. In addition, we have found that CD16+ DCs are the major producers of TNF-alpha and IL-6, while CD1c+ DCs produce primarily IL-8.

• molecule type
• lipopolysaccharide,
• Imiquimod,
• resiquimod,
• Pam3CysSK4,
• CpG,
• CD1c,
• CD141,
• flagellin,
• Interleukin-6,
• Tumor necrosis factor alpha,
• Toll like receptor,
• interleukin-8 (human),
• Polyinosinic acid:polycytidylic acid,
• CD16

• cell type
• myeloid dendritic cell,
• plasmacytoid dendritic cell

• experimental factor type
• Behavioural stimulus factor

created over 16 years ago (2 March 2009)    last modified over 12 years ago (4 June 2012)   [ RDF ]   [ RelFinder ]