...zation to the microtubules, are preferentially expressed in the CD4+ T cell subsets, suggesting they may share a common function in these cells. On the other hand, a different cluster of TRIMs is constitutively expressed at high levels in plasmacytoid dendritic cells (pDC). Conversely, he found that...

...uction by the myeloid and splenic CD8alpha+ and CD8alpha- DC. These data suggest that different pathogens may trigger differential cytokine production as a result of their engagement of distinct TLRs on DC, and in addition by their differential ability to induce IL-10 or IFN-alpha in different DC. T...

... generated data on the mechanisms for DC-induced Th1 development and the molecular pathways for inducing Th1 cells producing IFN-gamma solely, or Th1 cells producing IFN-gamma and IL-10. We have reported that plasmacytoid precursor dendritic cells (pDC) activated with the TLR-9 ligand CpG induce st...

...ociated molecular patterns and individual toll-like receptors on dendritic cells that turn on signal transduction pathways leading to the activation of different transcription factors. Microbial invasions are perceived by pattern recognition receptor (PRR)-expressing cells of the innate immune syst...

...ptides). To quantify presentation of the epitopes by DC, we used bulk T cells electroporated with TCR-encoding RNA in stimulation assays. Cytokine production by these T cells was used as a read-out. First experiments showed that loading of DC with the anti-DEC-205scFv-MAGE-A3-KKL construct led t...

...DC 05 trial (multipeptide loaded cytokine matured moDC + prior Treg elimination by 3x ONTAK treatment [5µg/kg], 8 evaluated pat.) did not show any enhanced immune reponses compared to the equivalent cohort in the DERMA-ER-DC 04 trial. Patient recruitment into the 2nd phase of the DERMA-ER-DC 06 t...

... MART-1 to transduce DC from melanoma patients. Biovex has developed HSV-based vectors optimised for antigen delivery to dendritic cells by the deletion of HSV genes which usually inhibit DC function. These deliver antigens to DC at very high efficiency and activate the transduced cells similarly t...

... signal after the fifth dose). This is indicative of T cell effector memory maturation (i.e., CD8+/CD28+/CD45RA- T cells) which are equipped to leave the peripheral circulation and infiltrate the tissues in surveillance of target antigens. It is also noteworthy that presence of CD28+ T cells in tumo...

...t 7 patients for which the analysis has been completed. Six of 7 patients developed CD8+ T-cell immune reactivity to several HIV antigens. Importantly, the expanded HIV-specific T cells are CD28+ effector memory cells, known to associated with long-term non-progression. The product was well-tolerat...

Data has been obtained from the application of a DC-based assay aimed at identifying new adjuvant molecules potentially capable to induce Th1 responses, determining the effects of new chemical compounds on dendritic cell capacity to produce type I IFNs. The selected molecules could be potential new ...

We annotated DC specific pathways and integrated them in the pathway tool. Initially we have annotated DC specific pathways using information publically available on the web. A preliminary curated non exhaustive pathway list can be found in the DC-THERA version of the program DC_Eu.Gene.

Data is available from a re-analysis of existing microarray data; we started experimental work to assess the reliability and completeness of the DC_Eu.Gene pathway set using stimulation of DCs with S.cerevisiae cells, pseudohyphae, spores in comparison with the results obtained with LPS, R848, LPS a...

...se the role of cell wall in the mechanisms of DC-Pathogen interaction as an experiment that could serve as reference in bioinformatics approaches for the reconstruction of DC activation signalling pathways. We have stimulated human Monocyte derived Dendritic Cells (hMoDC) with yeast, spheroplasts, p...

...of tumour antigen specific CD4+ T cell responses. We select the following tumour associated antigens: i) the E6 and E7 proteins of human papilloma viruses, ii) the carcinoembryonic antigen (CEA) and iii) the tumour specific antigen MAGE-A3. We investigated spontaneous CD4+ T cell responses to t...

...ecrete IL-2 after restimulation in concordance with low effector cytokine (e.g. IFN) and lack of cytotoxic mediator (e.g. perforin) expression (Frentsch et al., unpublished)). They might present an attractive candidate memory/effector CD8+ T-cells to be induced in treatments based on dendritic cell...

...ng the same GP33 specificity  1a) present either in the same or 1b) in normal mice; 2) or other LCMV peptide specificities. We thus could demonstrate that the rules established for TCR-Tg cells also apply to endogenous T cells i.e, all CD8 T cells behave similarly. This Ms. is submitted to pub...

...types, we yet found heterogeneity within some TEM subsets. Our current efforts involve a transition to 8 colours surface staining, and the evaluation of antigen-specific cell sets identified with MHC tetramers. For the later process, we initiated data collection of EBV, CMV and HIV specific T cell...

...ate to lymph nodes in the steady state. In contrast it was found that lymph nodes that drain sites of mature DC or adjuvant inoculation recruited L-selectin- CCR7- effector and memory CD8+ T cells. This cell recruitment required CXCR3 expression on T cells and occurred through high endothelial cells...

...tic and immune system as well as human specific infections in vivo. We established “human hemato-lymphoid-system mice” by transplanting human CD34+ cord blood cells into irradiated newborn Rag2-/-gc-/- mice, leading to de novo development of human B, T, and dendritic cells; formation of struct...

...mount an immune response (i.e. cytotoxic T cell proliferation, some control of EBV driven B cell proliferation, perforine and granzyme expressing T cell infiltration in B cell infected areas in lymphoid organs in situ), however, specific T cells could not be detected directly ex vivo by looking at t...