Mechanisms for DC-induced Th1 development and the molecular pathways for inducing Th1 cells producing IFN-gamma / IL 10

Our lab generated data on the mechanisms for DC-induced Th1 development and the molecular pathways for inducing Th1 cells producing IFN-gamma solely, or Th1 cells producing IFN-gamma and IL-10.

We have reported that plasmacytoid precursor dendritic cells (pDC) activated with the TLR-9 ligand CpG induce strong development of Th1 responses (Boonstra et al, 2003). In contrast, bone marrow-derived myeloid DC (BM-myeloid DC) and splenic CD8alpha+ and CD8alpha- DC, despite expression of TLR9 mRNA, were poor at directing Th1 responses when activated with CpG.

We have determined mechanisms for DC-induced Th1 development and the molecular pathways for inducing Th1 cells producing IFN-gamma solely, or Th1 cells producing IFN-gamma and IL-10, which have important implications for regulation of the immune response to eradicate pathogens with minimum pathology. This manuscript is now submitted for publication (Saraiva, O’Garra et al, 2007 – under review for Immunity). We have already determined that GATA-3 remodels the IL-10 locus specifically in T cells (Shoemaker et al, 2006) but are now also showing that in the absence of GATA-3 other signalling pathways and transcription factors may lead to induction of IL-10.

• cell type
• T cell,
• T helper cell 1,
• myeloid dendritic cell,
• plasmacytoid dendritic cell

• molecule type
• CD8,
• CpG,
• Interleukin-10,
• Toll like receptor 9,
• Interferon gamma,
• GATA-BINDING PROTEIN 3

• tissue type
• human bone marrow

• organ type
• human spleen

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]