Study of functional homogeneity by transcriptional screening of subsets of CD8 PBL

Our lab obtained data on functional homogeneity from a gene expression analysis at single-cell level for the expression of 8 effector genes from normal donors.

Until 2006 we subdivided CD8 PBL from normal donors in 14 different cell sets based in the expression levels and the co-expression of 5 different cell surface markers and studied functional homogeneity by screening cells from each subset at single-cell level for the expression of 8 effector genes (Blood, 2007). Although these studies allowed us isolate some homogeneous cell types, we yet found heterogeneity within some TEM subsets.

Our current efforts involve a transition to 8 colours surface staining, and the evaluation of antigen-specific cell sets identified with MHC tetramers. For the later process, we initiated data collection of EBV, CMV and HIV specific T cell sets.

• cell type
• T cell,
• peripheral blood lymphocyte

• organism type
• Epstein Barr virus,
• HIV-1,
• Cucumber mosaic virus,
• Homo sapiens

• molecule type
• CD8,
• Major histocompatibility complex

• experimental factor type
• Cell type factor

• experimental design type
• in vitro design experiment

created over 16 years ago (2 March 2009)    last modified over 13 years ago (23 January 2012)   [ RDF ]   [ RelFinder ]