Transcriptional and pathway analysis of human monocyte-derived Dendritic Cells stimulated with yeast, spheroplasts, pseudohyphae, spores, purified RNA and not purified RNA, LPS

We obtained transcriptional and pathway analysis data of human monocyte-derived DCs stimulated with yeast, spheroplasts, pseudohyphae, spores, purified RNA and not purified RNA and LPS.

We propose the role of cell wall in the mechanisms of DC-Pathogen interaction as an experiment that could serve as reference in bioinformatics approaches for the reconstruction of DC activation signalling pathways. We have stimulated human Monocyte derived Dendritic Cells (hMoDC) with yeast, spheroplasts, pseudohyphae, spores, purified RNA and not purified RNA, LPS. We have measured IL-6, IL-8, TNFalpha, IL-1beta, IFNgamma, IL-10, IL-12p70, IL-12p40. We are performing transcriptional analysis on the above mentioned samples, using the Genomics facilities at Genopolis (P32). The results are being analyzed with DC_Eu.Gene Analyzer and compared with other experiments in order to identify DC- and immunological pathway differentially affected by the different stimuli.

We have collaborated actively with Ugo D'Oro, Giorgio Napolitani, Federica Sallusto and Antonio Lanzavecchia. This integration of efforts and information is instrumental to the fruition of the genomics information in the field of dendritic cells research. Regarding the knowledge portal, we submitted microarray data and SOPS on the above mentioned experiments.

• molecule type
• Interleukin-12 p70,
• Tumor necrosis factor alpha,
• Interleukin-10,
• Interferon gamma,
• Interleukin-6 human,
• interleukin-8 (human),
• Interleukin-12 p40,
• Interleukin-1 beta

• organism type
• Homo sapiens

• cell component type
• cell wall

• stimulus type
• lipopolysaccharide,
• ribonucleic acids,
• spore,
• spheroplast,
• Pseudohypha,
• Saccharomyces cerevisiae

• experimental design type
• in vitro design experiment,
• stimulus or stress response experiment

created over 15 years ago (2 March 2009)    last modified over 12 years ago (7 November 2011)   [ RDF ]   [ RelFinder ]