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Stimulation of monocyte-derived DCs
Laboratory protocol
Different strains of Saccharomyces cerevisiae were cultured and collected in different conditions:
* different growth phase (exponential and stationary phase)
* different growth media (standard and promoting pseudohyphal growth)
* different cell form (spheroplast, spore and whole cell)
Monocyte-derived DCs were added at a final concentration of 5x105 cells/ml into 96-well plates. Serial diluition of yeast cells and preparations were added to the MoDCs. Cytokine accumulation was evaluated in the supernatants at 24h by ELISA, according to a standard protocol and it was measured at 450nm. The ELISA kit for IL-12 and IL-10 was from Biosource.
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Culturing of Saccharomyces cerevisiae
Different strains of Saccharomyces cerevisiae are cultured and collected in different conditions:
different growth phase (exponential and stationary phase)
different growth media (standard and promoting pseudohyphal growth)
different cell form (spheroplast, spore and whole cell)
-
Addition of monocyte-derived DCs
Monocyte-derived DCs are added at a final concentration of 5x105 cells/ml into 96-well plates.
-
Addition of serial dilution of yeast cells and preparations
Serial diluition of yeast cells and preparations are added to the MoDCs.
-
Evaluation of cytokine accumulation
Cytokine accumulation is evaluated in the supernatants at 24h by ELISA, according to a standard protocol and it is measured at 450nm.
- biomaterial type
- Biomaterial,
- cytokine,
- Saccharomyces cerevisiae
created over 16 years ago
(2 March 2009)
last modified over 13 years ago
(28 September 2011)
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