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Protocols  >  Laboratory protocol  >  Stimulation of monocyte-der...

Stimulation of monocyte-derived DCs

Laboratory protocol

Different strains of Saccharomyces cerevisiae were cultured and collected in different conditions:
* different growth phase (exponential and stationary phase)
* different growth media (standard and promoting pseudohyphal growth)
* different cell form (spheroplast, spore and whole cell)

Monocyte-derived DCs were added at a final concentration of 5x105 cells/ml into 96-well plates. Serial diluition of yeast cells and preparations were added to the MoDCs. Cytokine accumulation was evaluated in the supernatants at 24h by ELISA, according to a standard protocol and it was measured at 450nm. The ELISA kit for IL-12 and IL-10 was from Biosource.

Protocol Steps:
Stimulation of monocyte-derived DCs Graph


created over 10 years ago (2 March 2009)    last modified over 7 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]