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Datasets  >  preclinical study dataset  >  Multiplex analysis of human...

Multiplex analysis of human DC stimulated with microbial pathogen derivatives

preclinical study dataset

Data has been generated by multiplex analysis on the response of human MoDC to yeast, spheroplasts, pseudohyphae and spores. Human blood contains myeloid (mDCs) and plasmacytoid (pDCs) dendritic cells that differ in cytokine secretion pattern and responsiveness to microbial stimuli. We found that LPS-stimulated mDCs are able to induce up-regulation of co-stimulatory molecules on co-cultured pDCs. Likewise, CpG-stimulated pDCs activate co-cultured mDCs. The cross talk between these two populations of DC is very efficient since it can be observed at mDC/pDC ratio ranging from 1/10 to 10/1. Activation of the co-cultured population is restricted to up-regulation of co-stimulatory molecules since it does not involve cytokine secretion by the indirectly activated cells. The process is cell contact-dependent, although CD40-CD40L interaction is not involved, but it is also partly dependent on soluble factors. Indeed TNFa produced by LPS-activated mDCs plays a role in the activation of the co-cultured pDC. On the other hand type I interferons produced by CpG-activated pDCs is required for activation of the mDC. We also observed a strong synergistic response of both cell types, using limiting conditions of stimulus on co-cultured mDCs and pDCs.

The study on the response of human MoDC to yeast, spheroplasts, pseudohyphae and spores was performed in collaboration with Duccio Cavalieri's group (partner 15). Analysis of IL-6, IL-8, TNFalpha, IL-1beta, IFNgamma, IL-10, IL-12p70, IL-12p40 secretion was performed with a multiplex assay.
We will evaluate the capacity of MoDC stimulated by different forms of S. cerevisiae and Candida albicans to differentially induce T cell polarization.

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