...ents. We speculate that DC have adapted an endogenous compartment facilitating prolonged MHC class I presentation from exogenous antigen for effective CD8 T cell cross-priming. We have further analyzed the immunological consequences of the antigen depot. T cells that were induced by dendritic cell...

Our laboratory obtained data on the induction of immune responses after targeting antigens to DC via TLR agonists.

...sduction of cells that were found in draining lymph nodes (LNs) and induced potent anti-OVA cytotoxic T cells similar to those elicited by ex vivo transduced DC. The cytotoxic T-lymphocyte (CTL) response following direct injection of lentiviral vectors was highly effective in eliminating target cell...

...atients showing good CTL reponses (IFN-y Elispot) to multiple class I and II peptides compared to low responders of the stage III cohort. Additional in vitro maturation of DC by CD40L (leucine zipper, Immunex) did not result in better immunity compard to standard cytokine cocktail matured DC. The t...

...that DC can migraten efficiently to LN, the RNA was modified by introducing different RNA caps, introducing different 3’ UTRs, and by elongation of the poly A tail. Furthermore, mouse experiments have been started in collaboration with P02 to perform quantification of E/L-selectin-transfected DC t...

...g tetramer and cloning of the tetramer-positive lymphocytes. The result of these tests were considered positive if the post-immunization frequency of the different anti-TAA CTLp among the CD8+ T cells was 10-fold higher than the one encountered before vaccination. For both patients the tests were pe...

...cts were deficient in T cell IFN-gamma and IL-2 production pre-vaccination. The IL-2 defect was corrected in 7/12 patients post-vaccination. Vaccination resulted in an increase in tumor antigen-specific T cells in 8/12 patients. 7/12 subjects had a response to more than one antigen post-vaccinatio...

...rther data available on a clinical trial on vaccination of B-CLL patients with autologous, apoptotic, leukemic cells (Apo-DC). Cohort 1 with the vaccine alone and cohort 2 (5 patients) combining the vaccine together with GM-CSF as an adjuvant has finished accrual and all patients in these two cohort...

...lanning clinical trials of melanoma patients treated with DC and tumour lysate plus adoptive transfer of T cells which will result in corresponding data.

...tly ex vivo flow cytometric analysis hardly any CD8+ T cell response could be detected. The T cell cultures obtained from the biopsies taken of the last vaccination site were found to be HPV16 peptides specific in 3/5 tested NR patients and in 4/5 tested CR. However, recognition of the recombinant...

...ated the development of alternative therapeutic strategies. One of the approaches that has gained prominence in recent years is therapeutic vaccination. We decided to assess the capacity of mature dendritic cells, derived from blood monocytes of HIV-1 infected patients, to generate functional T-cell...

...tive factors for the response to IM pertain to the intrinsic features of GIST tumor cells but not to the host. Besides its direct antiproliferative activity on tumor cells, IM can trigger NK cell effectors which contribute to tumor regression in mice. Therefore, we addressed whether NK cell functi...

...7a and intracellular cytokine staining for the further analysis of individual T-cell populations and T-cell clones. Monitoring of the ongoing vaccination trial with RNA transfected autologous DC was done with overlapping peptides covering the whole protein sequence of the tumor antigens MageA3, Mel...

Our lab conducted an immunomonitoring study, such as on effector memory T cells, which led to corresponding immunological data.

...ggesting that high avidity was not part of the putative capability of these CTL to trigger tumor rejection. Most anti-MAGE-3.A1 CTL clones obtained after DC vaccination, but not after peptide or ALVAC vaccination, produced IL-10. Transcript profiling confirmed this result and indicated that about 20...

...ntify the target antigen recognized by the tumor reactive T cells in order to further improve T cell stimulations and to possibly complement treatment with DC vaccination. We then evaluated the capacity of autologous DC and HEK293 cells transfected with relevant HLA alleles to function as T cell t...

... the S-epimer of the Pam3CSK4 to long peptides containing a CTL epitope. We found that the Pam(R)-conjugates lead to better activation of dendritic cells than the Pam(S)-conjugates as judged by higher IL-12 secretion, up-regulation of relevant markers for dendritic cell maturation. In contrast, both...

Knowledge and gene expression data were gained on differentiation patterns of T cells.

...mas with later lung and liver metastases; the course of disease and the type of tumours that develop closely recapitulate many features of human breast cancer. A collaborative study has commenced between Austyn and Cerundolo to use this model to study new strategies of DC-based and other therapies f...

...ragment ions are matched against the calculated fragments of all tryptic peptides from the human sequences. Additional constraints were set by searches against a reversed human database to minimize false positive protein identifications. These criteria resulted in a list of 2794 unique proteins that...