...e are now aiming to improve the recipient mouse background by co-transplanting human mesenchymal stroma cells, and by adding human cytokines as well as human MHC. Furthermore, we use the mice to evaluate targeted therapies directed at human immune system cells as T cells, B cells, and dendritic cell...

We have data available on a novel M-CSF dependent DC developmental pathway that is independent of Flt3L. These DC have unique characteristics and precursor origin. The cells can be found in vivo in Flt3L gene deleted (-/-) mice injected with recombinant M-CSF.

Data has been obtained from an analysis of immature DC and mature DC from two strains of mice: the MyD88 deficient and the wild-type mice.

Our laboratory has obtained data on the kinetic of the transcriptional profiling of purified mouse DC treated with IL10.

We have generated data on the kinetic of the transcriptional profiling of D1 cells treated with either DMSO or LPS.

... DC subset called IKDC producing IFNg and expressing TRAIL which can recognize and kill transformed tumor cells in vitro and in vivo. They performed a video-confocal microscopy overnight on cultures of tumor cells with IKDC. We visualized that IKDC generate long contacts and synapses with tumor cell...

...actions of DC from crude population of untouched splenic cells with a purity of 70-80%. This includes several sophisticated steps for depletion of all unwanted cell types. The crude population of DC is divided into the three known splenic subsets (CD8+, CD4+, DN DC) using Fluorescence activated cell...

...as MRI data available on the migration of DCs in vivo in melanoma patients. From isolated lymphnodes obtained after surgery we could identify single DC after staining with Prussian blue for iron. We are in the process of analysing the T cell rosettes (activation stage) that surround these DC to get ...

...in order to understand their role in antigen presentation and their interaction with the regulation of translation in response to pathogens. We are still in the process of testing the use of translation monitoring by imaging technology as a novel way of defining DC activation.

We obtained data on antigen delivery by a vector with ovalbumin and CMVpp65 in mice or human, respectively. We used a MHC II presentation pathway targeting vector originally published by Wang et al. 1999. Proof of principle studies for antigen delivery have been performed with ovalbumin (murine stu...

...4+ T cell responses against pathogens or tumors in humans. Our goal was to generate peptide-MHC class II multimers to follow antigen-specific CD4+ T cells in immunized individuals. The study was a collaboration with Monica Moro in P. Dellabona’s lab. to study CD4+ T cell responses against pathogen...

We obtained data on anti-tumor responses in melanoma patients that were vaccinated with autologous DCs loaded with a combination of tumor Ag peptides. This study is a collaboration with the lab of Didier Colau and Thierry Boon in Brussels. HLA-DP4/MAGE3 molecules have been prepared to stain ex vivo ...

... respectively. Assays used have been 3H-thymidine-incorporation, ELISA, CBA, LDA and 51Cr-release assays. Autologous tumour specific CD8+ and CD4+ T cell clones have also been obtained from the blood and tumour infiltrating lymphocytes. We investigated the role of spontaneous CD4+ T cell responses a...

...T cell responses (thymidine-incorporation, ELISA, LDA and ELISPOT assays) using “conventional” techniques. We have set the staining conditions for the HLA-DR*1101 tetramers loaded with tetanus toxoid and MAGE-3 peptides corresponding promiscuous CD4+ T cell epitopes. Antigen-specific CD4+ T cell...

...Th-cells after in vitro or ex vivo stimulation. Since the entire population of antigen-specific Th-cells can be assessed in combination with intracellular cytokine analysis the methodology enables a combined quantitative and qualitative highly sensitive monitoring of Th-cell immunity (also after DC...

...CD8 multiple cell surface markers and functional profiles studied at single cell level. For that purpose, we subdivided peripheral CD8 T cells into eleven different cell subtypes based on the association of multiple cell surface markers. In each subtype, we isolated single-cells. In each single cell...

...nt cell types have a different distribution in the blood as compared to other lymphoïd organs. We characterized these subtypes in the blood by single-cell multiparametric analysis. Our preliminary results indicate these subtypes in the blood correspond to homogeneous different populations, but cell...

...ical trial of a DC vaccine administered into irradiated tumours in RCC. Three patients were enrolled and DC vaccine was produced. However only one patient received the vaccine due to cancer progression in the other two patients. The DC were labelled with 111In. The following patients will receive DC...

...iments DC-loaded with apoptotic leukemic cells were shown to induce a strong preferentially Th1 T-cell response. Apoptotic tumor cells were more effective than lysate, RNA and cell hybrids. A clinical trial has been initiated where autologous dendritic cells are loaded with apoptotic leukemic cell...

...ency of vaccine specific CTL in their peripheral blood and the appearance of a measurable CTL-frequency was seen between 8-13w after the first vaccine, furthermore, regression or stabilization of metastases has been coincident with the appearance of detectable or a significant rise (>10-fold) of CTL...