Infection of human Hemato-Lymphoid System Rag2gc-/- mice with both CXCR4 as well as CCR5 tropic HIV-1 strains

Data is available on human Hemato-Lymphoid System Rag2gc-/- mice infected with both CXCR4 as well as CCR5 tropic HIV-1 strains.

HIV causes a disseminated infection and spreads in all newly generated lymphoid tissues, thus closely resembling HIV infection in humans. However, as in EBV infected animals, adaptive T and B cell responses were not consistently detected.

Thus, although these “humanized” mice represent a major step forward in generating an in vivo preclinical testing system, they still harbor limitations as inconsistent/weak adaptive immune responses, questionable T (and B) cell selection, insufficient human hematopoietic stem cell maintenance, and myeloid cell constitution. To address these issues, we are now aiming to improve the recipient mouse background by co-transplanting human mesenchymal stroma cells, and by adding human cytokines as well as human MHC. Furthermore, we use the mice to evaluate targeted therapies directed at human immune system cells as T cells, B cells, and dendritic cells.

• organism type
• Mus musculus,
• HIV-1,
• Homo sapiens

• molecule type
• chemokine receptor CCR5,
• CX chemokine receptor 4,
• cytokine,
• Major histocompatibility complex,
• RAG2

• cell type
• T cell,
• mesenchymal cell,
• B cell,
• dendritic cell,
• myeloid cell

• tissue type
• stroma

• experimental design type
• in vivo design experiment

created over 16 years ago (2 March 2009)    last modified over 13 years ago (23 January 2012)   [ RDF ]   [ RelFinder ]