...with PolyI:C, pretreated or not with PP2, microarray analysis was performed. 41 genes were found to be strongly inhibited by PP2; some cytokines, chemokines and the transcription factor IRF-8 are among these genes. 38 genes were partially inhibited by PP2, and the IL-6 gene belongs to this second gr...

Studies have continued and data obtained on the effects of biolistic (Gene Gun) delivery of vectors engineered to express distinct chemokines at the local skin site of mice. In particular, attention has been focussed on the recruitment of distinct subsets of DC to skin. This work, which is being per...

... observed that simultaneous activation of TLR4 and TLR8 signaling cascades results in a marked inhibition of the secretion of the proinflammatory chemokine CCL2 with respect to stimulation through a single TLR. This inhibition is specific for both CCL2 and TLR agonist combination and could represent...

... reduced capacity to deliver activation and proliferative signals to gamma-delta T lymphocytes. Moreover, a dysregulated pattern of cytokines and chemokines produced by both cell populations is observed in the presence of the virus. Our preliminary results indicating that HIV-exposed MDDCs exhibit ...

...ccurs immediately after antigen stimulation, when cells able to induce inflammation are produced. We found a major expression of pro-inflammatory chemokines that is restricted to this period. We also developed an in vivo test where these effectors are transplanted in a matrigel, to study their capac...

We have data available on the role of chemokine receptors in regulating migration of mature DC and subsets of effector and memory T cells in secondary lymphoid organs. CCR7 is a chemokine receptor that is expressed by mature DCs and central memory T cells (TCM) and regulate migration of these cells ...

...s on the role of src-family tyrosine kinases in the maturation of human monocytes derived dendritic cells upon stimulation with several toll like receptor (TLR) agonists. The effect of these kinase inhibitors on the capacity of DC to be activated by a TLR2 (PAM3CSK4), TLR3 (Poly I:C), TLR5 (Flagell...

...bitory effect, including the expression of ILT-receptors and a panel of adhesion molecules known for their role in leukocyte migration are ongoing. Further studies are focussed on the dissection of the signal transduction pathway of 1,25(OH)2D3 in DCs, and on its interference with chemokine receptor...

...n of chemokine dependent CCR7 signalling, we have stimulated mature DCs with CCL19 or CCL21 for different time points. Western Blot analysis of phosphotyrosines showed a chemokine dependent and stimulation-time dependent phosphorylation pattern. We are now identifying and quantifying these chemokine...

To understand whether these regulators of gene expression could somehow contribute to the fine tuning of CCL2 expression in MD-DCs, a quantitative microarray approach that detects 723 human mature miR (miRBase 10.1, Dec 2007), was used to measure miR expression profile in these cells, either untreat...