Study of the role of src-family tyrosine kinases in the maturation of human monocytes

We obtained data using specific inhibitors on the role of src-family tyrosine kinases in the maturation of human monocytes derived dendritic cells upon stimulation with several toll like receptor (TLR) agonists. The effect of these kinase inhibitors on the capacity of DC to be activated by a TLR2 (PAM3CSK4), TLR3 (Poly I:C), TLR5 (Flagellin), and TLR8 (Poly U) agonist was evaluated. Src kinases were found to be required for the initiation of some maturation characteristics but not of others, since their inhibition was able to dissociate cytokines and chemokines production from the induction of surface maturation markers. Additional studies of the signal transduction pathways revealed that active src kinases are required for c-Jun phosphorylation and stabilization, induced by triggering of TLRs.

• molecule type
• toll-like receptor 5,
• chemokine,
• cytokine,
• Toll like receptor 2,
• Toll like receptor 3,
• Toll like receptor 8,
• Activator protein AP1 human,
• SRC human

• stimulus type
• ribonucleic acids,
• Pam3CysSK4,
• flagellin,
• Polyinosinic acid:polycytidylic acid

• organism type
• Homo sapiens

created over 15 years ago (2 March 2009)    last modified over 12 years ago (28 May 2012)   [ RDF ]   [ RelFinder ]