Gene expression in CD8 T cells in different inflammatory contexts

We obtained data from studies of CD8 T cell properties in man comparing HIV, CMV and EBV-specific cells.

It is usually assumed that effector gene expression in CD8 T cells may be similar in all immune responses, CD8 T cells in vivo differentiating into cytotoxic T cells that also produce g-inf. In contrast we published in 2007 that CD8 follow several differentiation waves and that effector gene expression is heterogeneous, different effector genes having different kinetics of expression-down regulation and associating randomly, suggesting that different immune responses could induce diverse differentiation patterns.

In the present year:
1° We studied the first differentiation wave, that occurs immediately after antigen stimulation, when cells able to induce inflammation are produced. We found a major expression of pro-inflammatory chemokines that is restricted to this period. We also developed an in vivo test where these effectors are transplanted in a matrigel, to study their capacity to attract other cell types.

2° We initiated a vast comparison between CD8 T cells responding to very different stimuli. Our preliminary results show that CD8 T cells responding either to virus or Listeria have very different properties, indicating that the inflammatory context defines CD8 T cells with different properties.

• molecule type
• peptide,
• CD8,
• chemokine,
• Interferon gamma

• cell type
• T cell,
• cytotoxic T lymphocyte

• organism type
• Viruses,
• Listeria

• experimental design type
• cell type comparison experiment

created over 15 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]