Monitoring of vaccine induced T cell responses for two clinical trials

We have obtained data from monitoring vaccine induced T cell responses for two clinical trials. We found CD8+ and CD4+ T cell responses against the immunological tracers KLH and TK, and against the NTPs and MAGE-3, respectively. Assays used have been 3H-thymidine-incorporation, ELISA, CBA, LDA and 51Cr-release assays. Autologous tumour specific CD8+ and CD4+ T cell clones have also been obtained from the blood and tumour infiltrating lymphocytes. We investigated the role of spontaneous CD4+ T cell responses against the HPV-18 and 16 E6 and E7 proteins in women with cervical lesions. When HPV-18 E6 was tested, we found a very high percentage of responders among women with high-grade cervical intraepithelial lesions, whose level of IFN gamma produced predicts the clinical outcome after surgery. We have continued the setting of the staining with HLA-DR*1101 tetramers loaded with promiscuous MAGE-3 peptides. We obtained specific staining by the use of peptides modified to contain only one predicted P1 anchor.

• molecule type
• CD8,
• melanoma antigen MAGE-3,
• HPV16 E6 long peptide,
• HPV16 E7 long peptide,
• Interferon gamma,
• CD4

• cell type
• T cell

• organism type
• Homo sapiens

• experimental design type
• ex vivo design experiment

created over 15 years ago (2 March 2009)    last modified over 12 years ago (2 April 2012)   [ RDF ]   [ RelFinder ]