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Monitoring of vaccine induced T cell responses for two clinical trials

clinical study dataset

We have obtained data from monitoring vaccine induced T cell responses for two clinical trials. We found CD8+ and CD4+ T cell responses against the immunological tracers KLH and TK, and against the NTPs and MAGE-3, respectively. Assays used have been 3H-thymidine-incorporation, ELISA, CBA, LDA and 51Cr-release assays. Autologous tumour specific CD8+ and CD4+ T cell clones have also been obtained from the blood and tumour infiltrating lymphocytes. We investigated the role of spontaneous CD4+ T cell responses against the HPV-18 and 16 E6 and E7 proteins in women with cervical lesions. When HPV-18 E6 was tested, we found a very high percentage of responders among women with high-grade cervical intraepithelial lesions, whose level of IFN gamma produced predicts the clinical outcome after surgery. We have continued the setting of the staining with HLA-DR*1101 tetramers loaded with promiscuous MAGE-3 peptides. We obtained specific staining by the use of peptides modified to contain only one predicted P1 anchor.





created over 14 years ago (2 March 2009)    last modified over 11 years ago (2 April 2012)   [ RDF Rdf ]   [ RelFinder Relfinder ]