Use of “conventional” techniques to monitor the vaccine induced specific anti-tumor T cell responses

We have obtained data monitoring the vaccine induced specific anti-tumor T cell responses (thymidine-incorporation, ELISA, LDA and ELISPOT assays) using “conventional” techniques. We have set the staining conditions for the HLA-DR*1101 tetramers loaded with tetanus toxoid and MAGE-3 peptides corresponding promiscuous CD4+ T cell epitopes. Antigen-specific CD4+ T cells are visualized after in vitro short-term expansion; we are currently optimizing the conditions for the ex-vivo staining. We have identified new MHC class II restricted epitopes on tumor associated antigens (i.e. MAGE-3, carcinoembryonic antigen and E6 and E6 transforming proteins of the human papilloma virus type 18) and we started to use them to follow the naturally occurring anti-tumor CD4+ T cells responses in patients suffering from melanoma, lung carcinoma and different stages of cervical intraepithelial neoplasia.

• cell type
• T cell

• molecule type
• carcinoembryonic antigen,
• peptide,
• Major histocompatibility complex class II,
• melanoma antigen MAGE-3,
• CD4

• organism type
• Human papillomavirus,
• Homo sapiens

• experimental design type
• in vitro design experiment,
• ex vivo design experiment

created over 15 years ago (2 March 2009)    last modified over 12 years ago (17 April 2012)   [ RDF ]   [ RelFinder ]