Following antigen-specific CD4+ T cells in immunized individuals

Data was obtained on CD4+ T cell responses against pathogens or tumors in humans. Our goal was to generate peptide-MHC class II multimers to follow antigen-specific CD4+ T cells in immunized individuals. The study was a collaboration with Monica Moro in P. Dellabona’s lab. to study CD4+ T cell responses against pathogens or tumors in humans. Soluble recombinant HLADR1101 MHC class II molecules receptive for loading with either pathogen or tumor-derived peptides were constructed. Although all these molecules could stain CD4+ T cells that had been stimulated for four days with peptide and IL-2, none of them could stain patients T cells ex vivo.

• molecule type
• peptide,
• Major histocompatibility complex class II,
• Interleukin-2,
• CD4

• cell type
• T cell

• organism type
• Homo sapiens

• experimental design type
• ex vivo design experiment

created over 15 years ago (2 March 2009)    last modified over 12 years ago (19 March 2012)   [ RDF ]   [ RelFinder ]