...hs after the last vaccination. At three months after vaccination there was a highly significant positive correlation between the strength of the interferon gamma and proliferative T cell response and the clinical response. In summary, clinical benefit was observed in 15 out of 20 patients (75%). A ...

...roporated with mRNA encoding 6 tumor-associated antigens, we persued this trial in a next cohort where DC-based vaccines were combined with low dose IFN-alfa (3 x 10e6 U per wk). In a significantly higher number of patients we observed vaccine-induced depigmentation and vitiligo. Further improveme...

...esting dosing or duration of IM administration (EORTC 62005 and BFR14 trials). “Immunological responders” were defined as patients whose NK cell IFN-gamma values after 2 months of IM were higher or equal to the baseline value at entry in the trial. The prognostic impact of IFN-gamma on progressi...

...rtain MAP kinases in the regulation of IL-10 and IL-12, IFN-gamma production. Using pharmacological inhibitors, or macrophages and DC from mice carrying a null mutation in MAP kinase signalling molecule(s), we have evidence for a role of certain MAP kinases in the regulation of IL-10 and IL-12, IFN...

...he lack of IFN-alpha production by the myeloid and splenic CD8alpha+ and CD8alpha- DC. These data suggest that different pathogens may trigger differential cytokine production as a result of their engagement of distinct TLRs on DC, and in addition by their differential ability to induce IL-10 or IFN...

...n the mechanisms for DC-induced Th1 development and the molecular pathways for inducing Th1 cells producing IFN-gamma solely, or Th1 cells producing IFN-gamma and IL-10. We have reported that plasmacytoid precursor dendritic cells (pDC) activated with the TLR-9 ligand CpG induce strong development ...

...with clear cell histology. As predicted from the in vitro data, the improved PME-CD40L DC product does indeed lead to restoration of both IL-2 and IFN-? responses. Also consistent with our in vitro observation is the gradual decline of the immune effector T cells in the peripheral circulation (i.e...

...olecules potentially capable to induce Th1 responses, determining the effects of new chemical compounds on dendritic cell capacity to produce type I IFNs. The selected molecules could be potential new vaccine adjuvants.

...lls (hMoDC) with yeast, spheroplasts, pseudohyphae, spores, purified RNA and not purified RNA, LPS. We have measured IL-6, IL-8, TNFalpha, IL-1beta, IFNgamma, IL-10, IL-12p70, IL-12p40. We are performing transcriptional analysis on the above mentioned samples, using the Genomics facilities at Genopo...

...ical lesions and we showed that the quality of the response (i.e. the level of IFN-? produced) could predict the clinical outcome after surgery. CEA specific CD4+ T cells were found both in normal donors and in patients but CD4+ T cells from the patients compared to normal donors showed impaired IFN...

The group has data available on a newly identified DC subset called IKDC producing IFNg and expressing TRAIL which can recognize and kill transformed tumor cells in vitro and in vivo. They performed a video-confocal microscopy overnight on cultures of tumor cells with IKDC. We visualized that IKDC g...

...hen HPV-18 E6 was tested, we found a very high percentage of responders among women with high-grade cervical intraepithelial lesions, whose level of IFN gamma produced predicts the clinical outcome after surgery. We have continued the setting of the staining with HLA-DR*1101 tetramers loaded with pr...

...iva et al., 2005). We have now shown that both IL-10 as well as IL-12 production is dependent on the signalling adaptor molecules either MyD88 or TRIF, respectively in response to CpG, LPS or PolyIC. This work has been published in the J.Immunology (Boonstra, Rajsbaum et al, 2006).

..., the biologically active metabolite of Vitamin D3, and its analogues, and its effect on the DC differentiation/activation pathway induced by type I IFN. 1,25(OH)2D3 prevented the generation of IFN-DCs when added to freshly isolated monocytes, and is capable to redirect already differentiated IFN-DC...

...and competitively inhibited LPS binding to the extracellular domain of TLR4. Addition of CyP together with LPS completely inhibited both MyD88- and TRIF-dependent pathways and suppressed the whole LPS-induced gene transcription program. CyP was effective in protecting mice from endotoxin shock in sp...

... T cell priming and/or NK cell triggering in HHD2 mice. We have used DC exosomes along with various TLR ligands or cytokines such as IL-2 or alpha IFN to promote Mart-1 specific T cell priming and/or NK cell triggering in HHD2 mice. We demonstrated that only TLR3 and 9 ligation is able to induce C...

...synthetic RNA containing 5’ phosphates. This form of activation is mediated by the helicase RIG-I and leads to production of high levels of type I IFNs. Notably, this observation may explain the ability of mRNA transfection to activate DC for immunisation protocols: such RNA, even if synthesised i...

... class II and produced cell type specific cytokines. CD8+ myeloid DCs “cross-presented” exogeneous Ags (Ovalbumin) while pDCs produce type 1 Interferons. The efficacy of CpG-DNA-Ag complexed for cross-presentation of Ag by DCs was analyzed in vivo. Primary and secondary CTL response against the ...

...pDC did not induce any detectable levels of cytokine. However, CpG stimulation of plasmacytoid pDC induced the cytokines IL-12p40, IL-12p70, TNF and IFN-alpha. Our data suggests that at this stage of infection plasmacytoid pDC may play only a limited role in protection against MTb infection. Our dat...

We have data available on dendritic cell development from hematopoietic stem and progenitor cells in steady-state and in inflammatory conditions, and, in particular, gene-expression data of Lin–c-KitintFlt3+M-CSFR+ cells in mouse bone marrow. Lymphoid tissue plasmacytoid and conventional dendrit...