In vitro activation of DC by transfection with single stranded viral or synthetic RNA containing 5' phosphates

We have obtained data from our studies demonstrating that DC and other cell types can be activated in vitro by transfection with single stranded viral or synthetic RNA containing 5’ phosphates. This form of activation is mediated by the helicase RIG-I and leads to production of high levels of type I IFNs. Notably, this observation may explain the ability of mRNA transfection to activate DC for immunisation protocols: such RNA, even if synthesised in the presence of cap analog, always contains a certain percentage of uncapped molecules bearing 5’ tri-phosphates.

• cell type
• dendritic cell

• organism type
• Viruses

• molecule type
• retinoid-inducible gene protein,
• type I interferon,
• messenger RNA

created over 16 years ago (2 March 2009)    last modified over 12 years ago (16 July 2012)   [ RDF ]   [ RelFinder ]