DC-development from hematopoietic stem and progenitor cells in steady-state and in inflammatory conditions

We have data available on dendritic cell development from hematopoietic stem and progenitor cells in steady-state and in inflammatory conditions, and, in particular, gene-expression data of Lin–c-KitintFlt3+M-CSFR+ cells in mouse bone marrow.

Lymphoid tissue plasmacytoid and conventional dendritic cells (DCs) are continuously regenerated from hematopoietic stem cells. The cytokine dependence and biology of plasmacytoid and conventional DCs suggest that regeneration might proceed through common DC-restricted developmental intermediates. By selecting for cytokine receptor expression relevant to DC development, we were able to identify highly cycling Lin–c-KitintFlt3+M-CSFR+ cells with a distinct gene-expression profile in mouse bone marrow that, on a clonal level in vitro and as a population both in vitro and in vivo, efficiently generated plasmacytoid and conventional DCs but no other lineages, which increased in number after in vivo injection of the cytokine Flt3 ligand. These clonogenic common DC progenitors (CDP) thus define a cytokine-regulated DC developmental pathway that ensures the supply of various DC populations in vivo.

• molecule type
• macrophage colony stimulating factor,
• Fms-related tyrosine kinase 3 ligand,
• Granulocyte macrophage colony-stimulating factor,
• type I interferon,
• signal transducer and activator of transcription 3,
• GATA-binding factor 1

• cell type
• MEP,
• granulocyte,
• erythrocyte,
• dendritic cell,
• megakaryocyte,
• plasmacytoid dendritic cell,
• macrophage,
• hematopoietic cell

• organism type
• Mus musculus,
• Homo sapiens

• tissue type
• human bone marrow

created over 16 years ago (2 March 2009)    last modified over 12 years ago (17 September 2012)   [ RDF ]   [ RelFinder ]