...btained transcriptomic data by analysing the effect of the inhibition of Src family kinases by the specific inhibitors PP1/2 on the uncoupling cytokine production in DCs stimulated by agonists of several Toll like receptors. Whole transcriptome analysis indicated the transcription factor IRF-8 and 3...

...ve data on a novel innate signalling pathway in DC. Using curdlan as a specific agonist of dectin-1, we have shown that this C-type lectin couple to Syk kinase leads to activation of ERK, JNK and p38 MAPKs, as well as NF-kappaB. However, signalling terminates rapidly once the receptor is internalise...

...tion of IL-12 p70, IL-10, and interferon-alpha was observed only at extremely high doses. Protein kinase R phosphorylation on transduction at an MOI of 150 was demonstrated by Western blotting. A Toll-like receptor (TLR)-driven luciferase reporter assay showed dose-dependent activation of TLR2, TLR3...

...ounds of mice, stimulated with CpG, in the presence or absence of a MEK inhibitor, and further data on the role of certain MAP kinases in the regulation of IL-10 and IL-12, IFN-gamma production. Using pharmacological inhibitors, or macrophages and DC from mice carrying a null mutation in MAP kinase...

...3A chain is the limiting component for the production of a complete IL-23 complex. Moreover, transcriptional analysis showed that the role of Src kinases on specific transcription factors accounts for unbalanced IL-12/IL-23 production. We will investigate if normal IL-23 production associated to i...