In vitro system to mature myeloid DCs and plasmacytoid DCs

Data was obtained using CpG-DNA as TLR9 ligand, to establish an in vitro system to mature myeloid DCs and plasmacytoid (p) DCs and on the efficacy of CpG-DNA-Ag complexes for cross-presentation of Ag by DCs was analyzed in vivo. Mature DCs upregulated CD80, CD86, MHC class II and produced cell type specific cytokines. CD8+ myeloid DCs “cross-presented” exogeneous Ags (Ovalbumin) while pDCs produce type 1 Interferons. The efficacy of CpG-DNA-Ag complexed for cross-presentation of Ag by DCs was analyzed in vivo. Primary and secondary CTL response against the C8 T cell epitope SIINFEKL was monitored using peptide-tetramer assays.

• molecule type
• deoxyribonucleic acids,
• CD8,
• Major histocompatibility complex class II,
• Ovalbumin,
• CpG,
• type I interferon,
• Toll like receptor 9,
• CD86,
• CD80

• cell type
• myeloid dendritic cell,
• plasmacytoid dendritic cell,
• cytotoxic T lymphocyte

created over 16 years ago (2 March 2009)    last modified over 12 years ago (6 August 2012)   [ RDF ]   [ RelFinder ]