Data has been obtained in a study of antigen recognition of blood-derived T cells. In this study, an IFNgamma ELISPOT was done with PBMC loaded with peptide pools, each consisting of 10 15-mers, which overlap with 11 aminoacids.

...gens was evaluated by CD137 upregulation, CD107a and CD40L expression using flow cytometry. Luminex technology was used to quantify the TNFalpha and IFNgamma secretion.

...of the molecular mechanisms responsible for NK cell activation by LPS-primed DC, we have found that three soluble DC-derived molecules, IL-2, type I interferons (IFNs), and IL-18, are necessary and sufficient to elicit efficient production of IFN gamma from NK cells. These analyses have been perform...

...servation strongly suggested that the molecules selectively produced by DC in presence of Th1 stimuli and not in presence of Th2 stimuli were type I IFNs and that these cytokines were presumably required for NK cell activation. In fact, our preliminary results confirmed this hypothesis with all of t...

Our laboratory has obtained data on the role of DC-derived cytokines in NK cell production of IFN-gamma.

...T cell activation. In fact the mDC/pDC cooperation event is able to significantly increase the ability of these cells to drive the proliferation and IFN? production by alloreactive T cells. In addition, we discovered that pDCs are not able to respond to whole fixed or live bacteria and consequently,...

...ndent on soluble factors. Indeed TNFa produced by LPS-activated mDCs plays a role in the activation of the co-cultured pDC. On the other hand type I interferons produced by CpG-activated pDCs is required for activation of the mDC. We also observed a strong synergistic response of both cell types, us...

... in the presence of GM-CSF and IL-4 (IL-4 DC), and antigen-stimulated circulating gamma delta T lymphocytes, bearing the Vgamma2 TCR. Co-cultures of IFN-DCs with zoledronate-stimulated gamma delta T lymphocytes were also performed. gamma delta T-cell activation, as demonstrated by their up-modulatio...

We obtained data on the influence of bLf, whose immunomodulant properties are now recognized, on MDDC differentiation/activation. We found that bLF interferes with the activation of MD-DC induced by different TLR agonists, but not by T cell mediated signals. Although bLF did not significantly influ...

...vaccination application. We have also initiated studies on the impact of dsRNA on the regulation of translation and the functional response of DCs (IFN production). We have now demonstrated that DCs enters an “unfolded protein response-like state and controls the phosphorylation of a key translat...

...lation of CD83 expression on human DC through RNA interference (RNAi) results in a less potent induction of allogeneic T cell proliferation, reduced IFN-gamma secretion by established T cells and decreased capacity in the priming of functional tumor antigen-specific CD8+ T lymphocytes. In addition, ...

...creased allostimulatory capacity and secretion of interleukin (IL)-6, IL-8, and tumor necrosis factor-alpha. Production of IL-12 p70, IL-10, and interferon-alpha was observed only at extremely high doses. Protein kinase R phosphorylation on transduction at an MOI of 150 was demonstrated by Western b...

...ve changes in DC migration and leads to their activation in lymph nodes but not lymph. These changes are due to TNF-alpha (migration) and Type 1 interferons (activation), but are not accompanied by obvious oral adjuvant effects. In contrast Etx causes only minor changes in migration and activation b...

My laboratory obtained data from a continued analysis of the ability of invariant NKT (iNKT) cells to assist priming of antigen specific T and B cell responses. We have carried out three complementary lines of research: 1) We have demonstrated that activation of human DC by Toll like receptor ligan...

We obtained data from studies of CD8 T cell properties in man comparing HIV, CMV and EBV-specific cells. It is usually assumed that effector gene expression in CD8 T cells may be similar in all immune responses, CD8 T cells in vivo differentiating into cytotoxic T cells that also produce g-inf. In...

...ration and a NKG2D-dependent activation resulting in an anti-metastatic effect. In humans, Dex bear functional IL-15R? which allow proliferation and IFNg secretion by NK cells. In contrast to immature DC, human Dex harbor NKG2D ligands on their surface leading to a direct engagement of NKG2D and NK ...

...ut is critical for blocking fatal immune pathology. In patients with chronic tuberculosis (TB), T cells are present that produce both IL-10 and IFN-gamma and stimulation of MTb-antigen specific T cells ex-vivo in the presence of anti-IL-10 antibodies results in their increased proliferation and IFN...

...C57Bl/6 mice, DC from TLR9 -/- produced little if any interferon alpha. However, MVA-BN induced IFNalpha in presence or absence of TLR9 -/-. Moreover, coinfection with MVA-BN and Ectromilia protected TLR9 -/- mice against death. Surprisingly, coinfection with MVA-BN and Ectromilia also protected IFN...

...asured in the blood with outcome, as there have been so far statistically significantly less events in stage III patients showing good CTL reponses (IFN-y Elispot) to multiple class I and II peptides compared to low responders of the stage III cohort. Additional in vitro maturation of DC by CD40L (l...

Summary of the completed RCC clinical study and the data obtained: RCC subjects were deficient in T cell IFN-gamma and IL-2 production pre-vaccination. The IL-2 defect was corrected in 7/12 patients post-vaccination. Vaccination resulted in an increase in tumor antigen-specific T cells in 8/12 p...