Identification of STAT-1 pathway in response of DC to Th1-inducing stimuli

We have obtained data on the STAT-1 pathway in response of DC to Th1-inducing stimuli. We have identified some genes differentially expressed in the different conditions. In particular, the most relevant gene upregulated in presence of stimuli typically inducing Th1 responses (LPS, CpG, Poly I:C) but not in presence of the Th2 stimulus, Pam3Cys, was Stat-1. We then evaluated whether STAT-1 was phosphorylated in DC following activation with TLR-dependent Th1 and Th2 stimuli and we found that only in presence of TLR stimuli able to induce Th1 responses STAT-1 was activated. This observation strongly suggested that the molecules selectively produced by DC in presence of Th1 stimuli and not in presence of Th2 stimuli were type I IFNs and that these cytokines were presumably required for NK cell activation. In fact, our preliminary results confirmed this hypothesis with all of the Th1 stimuli tested including the Poly I-C stimulus that mimics viral infections.

• molecule type
• CpG,
• Peptidylglycine alpha-amidating monooxygenase 3 Cys,
• STAT-1 gene,
• type I interferon,
• signal transducer and activator of transcription 1,
• Polyinosinic acid:polycytidylic acid

• cell type
• dendritic cell,
• natural killer cell

• stimulus type
• T helper cell 1,
• T helper cell 2

• experimental design type
• in vitro design experiment

• experimental factor type
• Stimulation by molecular entity factor

created over 15 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]