Studies of rat DC migration and activation in response to TLR agonists in relation to oral adjuvants

We have obtained data on the immuno-physiology of rat and mouse intestinal dendritic cells. We have used a known adjuvant, E. coli heat-labile toxin (Etx) and a potential adjuvant R-848, a small TLR7/8 ligand. We have shown that R-848 causes massive changes in DC migration and leads to their activation in lymph nodes but not lymph. These changes are due to TNF-alpha (migration) and Type 1 interferons (activation), but are not accompanied by obvious oral adjuvant effects. In contrast Etx causes only minor changes in migration and activation but is a potent oral adjuvant. Current work is centering on understanding the cellular and molecular bases of these differences and examining other TLR7/8 ligands.

• organism type
• Mus musculus,
• Rat,
• E. coli

• organ type
• human lymph node,
• intestine

• molecule type
• resiquimod,
• enterotoxin,
• Tumor necrosis factor alpha,
• type I interferon,
• Toll like receptor

• cell type
• dendritic cell

• experimental factor type
• Stimulation by molecular entity factor

created over 15 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]