Data has been obtained in a study of antigen recognition of blood-derived T cells. In this study, an IFNgamma ELISPOT was done with PBMC loaded with peptide pools, each consisting of 10 15-mers, which overlap with 11 aminoacids.

...the antigen recognition of DTH infiltrating T cells. To obtain the data, activation of CD4 and CD8 T cells upon recognition of EBV-B cells expressing vaccinal antigens was evaluated by CD137 upregulation, CD107a and CD40L expression using flow cytometry. Luminex technology was used to quantify the T...

Transcriptomic data has been generated on PB-monocytes in healthy controls, RA and SLE.

Data has been obtained on expression and regulations of Notch ligands on DC subsets.

...ntify new genes that change their expression at the cell surface during DC maturation, that may serve as novel maturation markers or that may tell us something new about DC maturation. From a more systems biology perspective, we will determine the amount of correlation between transcriptome and prot...

...ffort is put to understand the impact of transcriptome remodelling on phagosome proteome remodelling during DC maturation. This task requires the development of dedicated software solutions that are currently on the way and should open new possibilities for transcriptomic data analysis from the DC-T...

Data were obtained from rat DC subsets by transcriptional profiling.

...transcriptomic data by analysing the effect of the inhibition of Src family kinases by the specific inhibitors PP1/2 on the uncoupling cytokine production in DCs stimulated by agonists of several Toll like receptors. Whole transcriptome analysis indicated the transcription factor IRF-8 and 38 other ...

We generated data on the expression by dendritic cells treated with microbial pathogen derivatives.

...signing dendritic cell-based bioassays. To identify possible differentially expressed genes by DC after activation with stimuli typically involved in inducing Th1 or Th2 type responses we have performed comparative kinetic global gene expression analyses of DC activated with different TLR agonists t...

Gene expression data were generated on DC activated with different TLR agonists typically associated with the respective response.

...ating 3 times this analysis to put quantitative figures on the qualitative list of identified proteins. This list has been transferred to Duccio Cavalieri as part of the DC-ATLAS effort. They have identified several proteins of interest and are waiting to have a sufficient staffing to characterize t...

...the control of the responsiveness of antigen-presenting cells to LPS. Although these data still need independent validation, we would like to propose that DC need to down-regulate this inhibitory receptor upon encountering a maturation stimulus in order to be released from their immature state and t...

...cation of the DC subset proteome and the quantitation software. All our data can be accessed and analyzed through the MAPU database (Gnad et al.) After finishing our proteomic study of steady-state dendritic cell we will now start to analyze proteomic changes of DC subsets after different stimuli....

Our group has generated pathway data from human DC treated with pathogen derivatives.

...d motility of NK cells by tracking migration of DC-primed NK cells in vivo. Since LPS-activated DC are potent inducers of NK cell priming and lymph nodes appear to be a key place in which DC and NK cell interactions occur, we have investigated the in vivo capacity of resting and DC-primed NK cells t...

...that ICAM-1 expression by mature DCs is critical for long-lasting contacts with CD8+ T cells, but dispensable for short-lived antigen-specific interactions. Serial brief T cell-dendritic cell contacts induced early CD8+ T cell activation, proliferation and effector CTL differentiation in the first f...

...ing of translation) to monitor translation by FACs in individual or cell populations. We have demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. We hope that this technology will become rapidly popular among the DC-THERA participant...

Flow cytometric analysis data were obtained of translation activation in DC-activated T cells.

We have obtained data by fluorescence microscopy analysing DC distribution in spleen and how this is affected during inflammation driven by stromal cells or hematopoietic cells. One of our images of DC was selected as the cover for the June 2007 issue of Nature Immunology.