Confocal microscropic analysis of translation activation in DC-activated T cells

We have obtained imaging data by confocal microscopy of translation activation in DC-activated T cells and have pursued our efforts on the definition of a novel way of monitoring DC and T cell activation as well as studying viral infections. We also have now applied the puromycin-based technology developed in our laboratory (SUnSET, surface sensing of translation) to monitor translation by FACs in individual or cell populations. We have demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. We hope that this technology will become rapidly popular among the DC-THERA participants.

• cell type
• T cell,
• dendritic cell

• organism type
• Viruses

• molecule type
• puromycin

• experimental design type
• observation design

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]