... in vitro, and in vivo when the cells were implanted into the chicken embryo CAM assay. VEGF production by DC was selectively observed only when the DC where alternatively activated. Therefore, VEGF production by DC can be considered a signature of this state of activation. AA-DC induced in the pres...

We have obtained data from our studies demonstrating that DC and other cell types can be activated in vitro by transfection with single stranded viral or synthetic RNA containing 5’ phosphates. This form of activation is mediated by the helicase RIG-I and leads to production of high levels of type...

...heless, mRNA-electroporated DC induce an effective cytotoxic T lymphocyte (CTL) response in vivo. Mature electroporated DC are significantly more potent in eliciting an Ag-specific CD8+ CTL response compared to their immature electroporated counterparts. In addition, a significant improvement in CTL...

... and produced cell type specific cytokines. CD8+ myeloid DCs “cross-presented” exogeneous Ags (Ovalbumin) while pDCs produce type 1 Interferons. The efficacy of CpG-DNA-Ag complexed for cross-presentation of Ag by DCs was analyzed in vivo. Primary and secondary CTL response against the C8 T cell...

We are continuing our studies of the roles of DC in scrapie uptake and dissemination and have obtained data on intestinal DC subsets all of which can acquire scrapie ME7 form the intestinal lumen and transport it via lymph. In current studies we are examining the effects of indomethacin-induced sm...

...ate progressive versus regressing P815 mastocytoma in order to identify cells which display immunosuppressive properties. We found changes in regulatory T cells populations as well as in the “myeloid suppressor cells”. Our preliminary data suggest that two populations of regulatory T cells coexi...

...that regulatory T cells have a stronger impact on immune responses against weakly immunogenic or auto-antigen (P1A). Treg are detected in growing P815 tumor and there is some evidence that 2 regulatory T cells develop sequentially. T cell activation was monitored by tetramer staining and in vivo CTL...

...as obtained on the contribution of pDC in acute MTb infection. We examined whether plasmacytoid pDC are infected and/or activated by MTb, and whether they play a role in regulating bacterial clearance during acute MTb infection and if so how. We have compared these DC with myeloid DC and macrophage...

...e tumor cells express the cognate CTL antigen. In tumors that do not express the CTL cognate antigen, we observe peripheral infiltration by CTLs, but the lymphocytes neither stop moving, nor kill tumor cells or infiltrate tumors deeply. Antigen expression by tumor cells therefore determines both CTL...

...ing DC which expressed a CD8?- CD11b+ F4/80+ gp40- surface phenotype. As demonstrated by electron microscopy some DC contained live amastigotes and expressed I-Ad/LACK complexes in the membrane of the parasitophorous vacuole. Therefore, LACK could be processed and presented to T cells by infected DC...

...ize the DC which carried I-Ad/LACK complexes at the cell surface in LACK-immunized BALB/c mice. Two different populations of DC, could thus be identified both expressing high levels of CD11c and MHC class II complexes but different levels of CD11b, suggesting that both Langerhans cells and dermal DC...

... for stimulating CD4 and CD8 T cell responses with the transfected DCs, matured for 2 days in poly I:C. The activation status of CD4+/CD25+ T reg in TIL in prostate cancer has been analysed. A higher frequency of CD4+/CD25+/GITR+/ICOS+/FoxP3+ cells in the areas with malignancy as compared to normal ...

...vent tumors from growing out. By depleting CD4+ and CD8+ cell populations prior to tumor challenge, CD8+ cells were identified as the main effector cells involved. Importantly, DCs pre-loaded in vitro with ICs are at least 1000-fold more potent than ICs injected directly into mice or DCs loaded with...

...ng peptide conjugates in vivo by either direct vaccination or ex vivo DC loading prior to vaccination. Both approaches show similar effectiveness in CTL priming suggesting that TLRL-peptide conjugates efficiently target DC in vivo.

... division of naive CTL in tumor draining lymph nodes, it does not establish a productive immune response. Treatment of tumor-bearing mice with dendritic cell-stimulating agonistic anti-CD40 mAb resulted in systemic efflux of CTL with robust effector function capable to eradicate established tumors. ...

...ll as endogenous polyclonal T cells, specific for the same antigens, identified by MHC pentamers loaded with specific peptides. In each individual T cell, we studied the expression of twenty different genes either mediating effector functions, or coding for different receptors involved in T cell dif...

... available on dendritic cell development from hematopoietic stem and progenitor cells in steady-state and in inflammatory conditions, and, in particular, gene-expression data of Lin–c-KitintFlt3+M-CSFR+ cells in mouse bone marrow. Lymphoid tissue plasmacytoid and conventional dendritic cells (DC...

... treatment with rIFNalpha at birth induced both FL and plasmacytoid DC (pDC) that resulted in enhanced resistance against HSV-1 at day 7. In contrast, in mice lacking FL, IFNalpha treatment at birth did not influence splenic cell composition and had no effect on viral protection. The transfer of pDC...

...+Foxp3+ regulatory T cells (Treg), ii) markedly enhance the magnitude of secondary but not primary CTL responsesinduced by DEX vaccines, iii) synergize with DEX in therapy but not prophylaxis tumor models. Therefore, therapeutic vaccines such as DEX aimed at boosting tumor-primed effector T cell...

... iNKT cells in vivo with the synthetic CD1d ligand alpha-GalCer significantly enhances immune responses to protein and peptide based vaccines, due to rapid iNKT dependent DC maturation. vi) We have also described the impact of combining injection of protein vaccines with a combination of iNKT cell...