Gene expression in T cells in responses to LCMV-GP33 protein and listeria-Ovatranscriptomic dataset
In our lab, we have gene expression data in T cells available in responses to LCMV-GP33 protein and listeria-Ova.
We studied TcR-Tg clones specific of each antigen (P14-GP33 specific, 0T-1-OVA specific), as well as endogenous polyclonal T cells, specific for the same antigens, identified by MHC pentamers loaded with specific peptides. In each individual T cell, we studied the expression of twenty different genes either mediating effector functions, or coding for different receptors involved in T cell differentiation and memory generation. We were surprised to observe that each type of infection induced peculiar expression of the effector molecules or receptors expressed by responding cells, thus generating unique divergent differentiation patterns. Endogenous LCMV polyclonal T cells and P14 T cells showed the same differentiation patterns that were different from those of OT-1 T cells responding to Listeria-OVA. These results suggest that CD8 differentiation is dependent of the infectious context, rather than of the TCR specificity.
Our present studies aim to confirm this notion. For that purpose, we will challenge P14 LCMV-GP33 specific T cells with the Listeria-GP33, to identify if these cells now express a pattern of differentiation similar to OT-1 T cells responding to Listeria-OVA. Whatever the mechanisms, these results describe for the first time alternative pathways of CD8 differentiation, that have nothing in common with those of CD4 T cells, since divergent gene expression does not mimic at all Th1 and TH2 differentiation.
- molecule type
- gp33 peptide,
- Major histocompatibility complex,
- T cell receptor,