We have obtained data on the immunogenicity induced by chemotherapeutics....

...ulating mature B cell compartment, whereas B cell development was unaffected by bmDC ablation. This phenotype could be reverted by B cell over-expression of the anti-apoptotic factor bcl2. Moreover, we showed that re-circulating B cell survival requires the presence of bmDC, which produce macrophage...

Data was generated on DC activation by different forms of the non-pathogenic yeast Saccharomyces cerevisiae. A special focus was laid on the response of MDDCs to the non pathogenic fungi.

...ce renal cell carcinoma and melanoma from this trial using cellular immunotherapy with dendritic cells loaded with tumor antigens. This study is not yet open for participant recruitment. ClinicalTrials.gov Identifier: NCT00610389 Purpose Background: cellular immunotherapy with dendritic cells (DC...

Transcriptional data is available on macrophage knockout BALB/c and C57BL/6 mice using different stimuli at different time points in the presence or absence of a kinase inhibitors.

...al relevance of the direct Treg-CD8+ T cell contacts that we imaged. Besides, Treg inactivation also induces an increase in the number of dendritic cells (DCs) in these tumor-draining lymph nodes. Therefore Tregs could influence CD8+ T cell arrest by modulating the number of antigen presenting cells...

... by FACs during DC/T cell interaction in presence or absence of cognate antigenic peptides. It was demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. Surprisingly, this phenomenon was mTOR independent (Rapamycin insensitive).

Data is available from an evaluation of different stimulation conditions for the cytometric assessment of antigen-specific Th-cells based on CD154 expression induced during short-term in vitro activation, a method established by us during previous DC-THERA activities. This is important and essential...

...t a novel C-type lectin, DNGR-1, can be used to mark DC upon injection into mice and might therefore be useful for in vivo imaging studies. Microscopy will continue to be used to understand molecular dynamics in cell biology and immunology, including studies of signalling pathways in dendritic cell...

Work has continued to characterise the function of a novel tick-derived DC modulator that perturbs various signalling pathways of human DC but not others; transcriptional profiling and pathways-based analysis will be undertaken in the next phase and data will become available.

Concerning the pathway based analysis and database we have started the curation of the TLR4-CD14 pathway in DCs and we have described the events occurring at the DC membrane level. We have also started global gene expression analyses to better difine the role of NFAT in DCs.

...microarray and RT-PCR data on the priming of CD8+ T cells in mice and their regulation of proliferation and development into effector and/or memory cells. The positive influence of a reactive lymph node on the priming of CD8+ T-cells was already analyzed in 2007. We set up experiments to determine ...

We obtained data on MHC II transport during human monocyte-derived DC maturation. We have published that the E3-ubiquitin ligase MARCH I is responsible for peptide-loaded MHC II ubiquitination and their subsequent internalization in immature DC.s The ligase MARCH I has been shown to be down-regulate...

...ailable from MRI tracking of dendritic cells in melanoma patients for cellular therapy. In vivo magnetic resonance tracking of magnetically labeled cells is feasible in humans for detecting very low numbers of dendritic cells in conjunction with detailed anatomical information. MRI cell tracking usi...

Data will be obtained from a study that we plan on DC receptor characteristics and signalling, focussing on antigen receptors.

... the proteome of the phagocytic/endocytic compartments that are formed upon cell entry of antigens via specific pathways. The ultimate goal is to get a better insight into the protein complexes that are involved in antigen processing, and learn whether the different entry routes for antigens into DC...

A large reference ‘golden experiment’ will be carried out in which the transcriptome of DC vaccines of 50-100 patients will be compared and data transcriptomic data will become available.

We will start a collaboration with G. Schuler (P06) to examine the modulation of dendritic cell function by Denileukin Difitox (ONTAK) and will provide pathway analysis data.

...essed primarily in mouse CD8?+ DC and their putative human equivalents. This lectin, named DNGR-1, can serve as a receptor for antigen targetting to DC. Notably, tumour antigens coupled to anti-DNGR-1 antibodies and given together with a suitable adjuvant elicit potent CTL responses that can promote...

We have used agonists of the dectin-1/Syk pathway as adjuvants in vivo to induce CD8+ T cell responses and have data available on this. In summary, we have shown that this can result in CTL capable of destroying tumours.