...okines on DC in vivo. Our investigation has shown that, in the absence of additional stimuli, such inflammatory signals are ineffectual in promoting DC activation and cannot substitute for engagement of innate receptors directly on DC.

...nct and restricted sets of cell surface markers and functional parameters. We obtained coexpression data of 18 genes simultaneously in individual cells from CD8+ T cell subsets (associated to multiple markers to subdivide CD8+ T cells into 14 different cell types, several of which were not describ...

...tion of the dsRNA with tumor antigen encoding mRNA can lead to the induction of a cytotoxic T-lymphocyte (CTL) response. Electroporation of immature DCs with poly(I:C(12)U), a dsRNA analogue, resulted in phenotypic as well as functional changes, indicative of DC maturation. Co-electroporation of DCs...

... antigen-specific CD8+ T lymphocytes. In addition, CD83 mRNA-electroporated DC are stronger T cell stimulators. However, CD83 overexpression on Melan-A/MART-1-specific tumor-infiltrating lymphocytes (TIL) circumvents the need for CD83 expression on DC. Co-culture of immature DC with TIL or K562 cell...

...ure monocyte-derived dendritic cells after transduction with high doses of lentiviral vectors. Dendritic cells (DCs) are an attractive tool for immunomodulation, targeting mature DCs (mDCs) for immunization or immature/semimature DCs (iDCs) for tolerization. Therefore, introducing antigens into DC...

...tigen (Ag) to deliver its cargo to endosomes of murine Dendritic cells (DCs), thus initiating TLR mediated DC maturation as well as processing of MHC class I and class II restricted epitopes. In these studies we used as Ag either Ovalbumin, or rec. Prion-protein. In the latter system, a CD4 T cell...

...of LPS stimulation, p50 -/- DC that are still alive (80% vs 40% of WT DC) display higher expression of MHC molecules and CCR7, as well as higher secretion of pro-inflammatory cytokines such as IL-1beta, TNF-alpha and IL-18. This correlates with the enhanced capability of p50-/- DC to activate T cell...

...opic as sites of inflammation are often characterized by both low oxygen tension and an extensive infiltration of inflammatory leukocytes, including DC, which need to move against oxygen gradients. We showed that oxygen availability is necessary to promote full monocyte-derived DC differentiation an...

We have obtained data on the immuno-physiology of rat and mouse intestinal dendritic cells. We have used a known adjuvant, E. coli heat-labile toxin (Etx) and a potential adjuvant R-848, a small TLR7/8 ligand. We have shown that R-848 causes massive changes in DC migration and leads to their activat...

...ctivation of naïve and memory T cells in rats. We have shown that two of the three DC subsets are highly potent activators of naïve CD4+ T cells, and do not appear to be constitutively suppressed in any way. We are currently examining the phenotype of the activated cells and determining if Treg...

...intestinal denritic cells are not constitutively biased towards stimluating Th2 responses (Milling). We have shown that antigen given orally during intestinal inflammation stimulates a strong response rather than tolerance.(Sebti). We have clarified the roles of dendritic cells and regulatory T cell...

...ted by activated iNKT cells (Hermans et al, 2007). DC maturation induced in vivo with any one of a variety of TLR ligands was greatly improved through simultaneous administration of the iNKT cell ligand alpha-galactosylceramide. DC isolated from animals treated simultaneously with TLR and iNKT cell...

We have obtained data from an analysis of antigen loading of DCs via targeting DC-SIGN, which resulted in enhanced class II-mediated presentation. Constructs for fusion proteins of the DC specific receptor DC-SIGN fused to GFP have been generated.

Our lab obtained data on maturation of DC by TLR agonists.

...e significant progress using biodegradable polymers to enhance cross-presentation of loaded antigens, and the best results were obtained by maturing DCs with a combination of the TLR ligands R848 and polyI:C. Furthermore, we have shown that DC electroporated with melanoma antigen RNA express the pro...

.../O and mutant mouse strains. Cross presentation is the process by which Dendritic cells (DC) phagocytose pathogens or dying cell fragments, and present proteolytic peptides derived from these antigens in association with MHC class I molecules. The reasons why DCs are the only antigen presenting cell...

...ence of TGF-beta during lactation and was mediated by regulatory CD4+ T lymphocytes. Adoptive transfer experiments of LACK-specific TCR transgenic T cells into newborn mice breastfed by LACK-exposed mothers demonstrated that LACK was readily processed and presented to T cells in the neonate. We the...

...om studies of CD8 T cell properties in man comparing HIV, CMV and EBV-specific cells. It is usually assumed that effector gene expression in CD8 T cells may be similar in all immune responses, CD8 T cells in vivo differentiating into cytotoxic T cells that also produce g-inf. In contrast we publis...

...unraveled the bioactivity of Dex onto NK cells, Dex promoting a IL-15Ra-dependent NK cell proliferation and a NKG2D-dependent activation resulting in an anti-metastatic effect. In humans, Dex bear functional IL-15R? which allow proliferation and IFNg secretion by NK cells. In contrast to immature DC...

...regulate macrophage antimicrobial activity or the induction of Th1 responses by dendritic cells (DC). However, during infection with pathogens such as T. gondii, IL-10 hinders pathogen clearance but is critical for blocking fatal immune pathology. In patients with chronic tuberculosis (TB), T cell...