... speculate that DC have adapted an endogenous compartment facilitating prolonged MHC class I presentation from exogenous antigen for effective CD8 T cell cross-priming. We have further analyzed the immunological consequences of the antigen depot. T cells that were induced by dendritic cells severa...

Our laboratory obtained data on the induction of immune responses after targeting antigens to DC via TLR agonists.

...of cells that were found in draining lymph nodes (LNs) and induced potent anti-OVA cytotoxic T cells similar to those elicited by ex vivo transduced DC. The cytotoxic T-lymphocyte (CTL) response following direct injection of lentiviral vectors was highly effective in eliminating target cells in vivo...

...ge III patients showing good CTL reponses (IFN-y Elispot) to multiple class I and II peptides compared to low responders of the stage III cohort. Additional in vitro maturation of DC by CD40L (leucine zipper, Immunex) did not result in better immunity compard to standard cytokine cocktail matured DC...

...iently transfected DC with RNA encoding a functional protein (E/L-selectin), which allows entry of DC into LN from HEV. These DC rolled in vitro on sialyl-LewisX-coated slides, and in vivo, mouse E/L-selectin-transfected DC homed to LN after i.v. application. Monocyte-derived and cocktail-matured DC...

...anoma Patients”. Patients received sequential immunizations with autologous dendritic cells pulsed with 8 HLA-restricted HLA-A2 peptides and a MAGE-3.DP4 peptide. They were randomized to receive immunizations either with I3 DC (DC generated with interferon-beta and interleukin-3) or with G4 DC (DC...

Summary of the completed RCC clinical study and the data obtained: RCC subjects were deficient in T cell IFN-gamma and IL-2 production pre-vaccination. The IL-2 defect was corrected in 7/12 patients post-vaccination. Vaccination resulted in an increase in tumor antigen-specific T cells in 8/12 p...

...cohorts that combine Apo-DC vaccination with leukemia debulking therapy is currently being considered for submission to the Swedish Medical Products Agency. Immunological monitoring in these patients involving leukemia-specific lymphocyte proliferation and ELISPOT assays, phenotyping of immune cell...

We are planning clinical trials of melanoma patients treated with DC and tumour lysate plus adoptive transfer of T cells which will result in corresponding data.

...E6 and E7, whereas CD8+ T cells were found merely against HPV16 E6 and to a less broad array of epitopes within each patient. This analysis was done after one in vitro restimulation, however, by directly ex vivo flow cytometric analysis hardly any CD8+ T cell response could be detected. The T cell...

...rgeting sequence of DC-LAMP. Broadly directed HIV-specific CD4(+) and CD8(+) cytotoxic T cells exhibiting a poly-functional cytokine secretion pattern were generated by co-culturing with autologous chimeric mRNA electroporated dendritic cells. Thus, administration of ex vivo generated dendritic cell...

...ferative activity on tumor cells, IM can trigger NK cell effectors which contribute to tumor regression in mice. Therefore, we addressed whether NK cell functions could be independent prognostic factors predicting long term survival to IM therapy. Methods: The functional phenotype of NK cells was...

...D FACSCanto II. Additionally we used functional markers such as CD107a and intracellular cytokine staining for the further analysis of individual T-cell populations and T-cell clones. Monitoring of the ongoing vaccination trial with RNA transfected autologous DC was done with overlapping peptides c...

Our lab conducted an immunomonitoring study, such as on effector memory T cells, which led to corresponding immunological data.

...1 T cells could initiate a tumor regression process. To understand the role of these T cells, we performed a functional analysis of anti-MAGE-3.A1 CTL clones derived from vaccinated patients who displayed tumor regression. The functional avidities of these CTL clones, evaluated in lysis assays, we...

...ges would be optimal to screen for antigen specificity in the patients T cells. However, during a second T cell culture to expand large numbers of T cells for this screening procedure a drastic expansion of regulatory T cells was observed. Since no sufficient number of T cells could be expanded the ...

...K4 or the S-epimer of the Pam3CSK4 to long peptides containing a CTL epitope. We found that the Pam(R)-conjugates lead to better activation of dendritic cells than the Pam(S)-conjugates as judged by higher IL-12 secretion, up-regulation of relevant markers for dendritic cell maturation. In contrast,...

Knowledge and gene expression data were gained on differentiation patterns of T cells.

...n and Cerundolo to use this model to study new strategies of DC-based and other therapies for advanced breast cancer and for which funding has now been secured for 3y from MRC UK. [A postdoctoral scientist was recruited for this Project from an Associated Partner – Josien, Nantes FR – via the DC...

...alpha, PGE2; 48 hours) monocyte derived human dendritic cells (DC) from a healthy donor has been established by using high accuracy, high sensitivity protein identification technology. We have identified 2794 proteins in DCs by liquid chromatography tandem mass spectrometry. Prior to MS analysis, DC...