Data has been obtained in a study of antigen recognition of blood-derived T cells. In this study, an IFNgamma ELISPOT was done with PBMC loaded with peptide pools, each consisting of 10 15-mers, which overlap with 11 aminoacids.

...een obtained on the antigen recognition of DTH infiltrating T cells. To obtain the data, activation of CD4 and CD8 T cells upon recognition of EBV-B cells expressing vaccinal antigens was evaluated by CD137 upregulation, CD107a and CD40L expression using flow cytometry. Luminex technology was used t...

Transcriptomic data has been generated on PB-monocytes in healthy controls, RA and SLE.

Data has been obtained on expression and regulations of Notch ligands on DC subsets.

...ith previously collected microarray data. From a purely immunological perspective, we will identify new genes that change their expression at the cell surface during DC maturation, that may serve as novel maturation markers or that may tell us something new about DC maturation. From a more systems...

...ed in various conditions. This methodology has been applied successfully to identify: a) a set of proteins specifically associated to phagosomes in DCs but not other phagocytes, such as macrophages, b) a set of proteins specifically recruited to phagosomes during DCs maturation induced by various ...

Data were obtained from rat DC subsets by transcriptional profiling.

...tomic data by analysing the effect of the inhibition of Src family kinases by the specific inhibitors PP1/2 on the uncoupling cytokine production in DCs stimulated by agonists of several Toll like receptors. Whole transcriptome analysis indicated the transcription factor IRF-8 and 38 other genes inc...

We generated data on the expression by dendritic cells treated with microbial pathogen derivatives.

...ained using Affymetrix mouse chips by designing dendritic cell-based bioassays. To identify possible differentially expressed genes by DC after activation with stimuli typically involved in inducing Th1 or Th2 type responses we have performed comparative kinetic global gene expression analyses of DC...

Gene expression data were generated on DC activated with different TLR agonists typically associated with the respective response.

...t of proteins present in macrophages lysosomes (in collaboration with M. Desjardins, Uni of Montreal). They hope to distinguish specific features of DCs to further understand the antigen processing and presentation abilities of these cells in response to pathogens. Meanwhile, they have finalized t...

...ng DC, induced by a prototypical maturation stimulus such as LPS. Results obtained from the LC-MS/MS analysis of the integral plasma membrane proteome of D1 cells stimulated for 24 hours with LPS have been compared to the results obtained from immature D1 cells. As expected, DC up-regulated the cell...

...r required SILAC (stable isotope labelling with amino acids in cell culture) labelling leaving rare cell populations, like in vivo DC subsets, out of reach. In this work package we introduced a robust and generally applicable quantification technology, which allowed us to quantify the dendritic cell...

Our group has generated pathway data from human DC treated with pathogen derivatives.

... and motility of NK cells by tracking migration of DC-primed NK cells in vivo. Since LPS-activated DC are potent inducers of NK cell priming and lymph nodes appear to be a key place in which DC and NK cell interactions occur, we have investigated the in vivo capacity of resting and DC-primed NK cell...

...by mature DCs is critical for long-lasting contacts with CD8+ T cells, but dispensable for short-lived antigen-specific interactions. Serial brief T cell-dendritic cell contacts induced early CD8+ T cell activation, proliferation and effector CTL differentiation in the first few days after immunizat...

...by confocal microscopy of translation activation in DC-activated T cells and have pursued our efforts on the definition of a novel way of monitoring DC and T cell activation as well as studying viral infections. We also have now applied the puromycin-based technology developed in our laboratory (SUn...

Flow cytometric analysis data were obtained of translation activation in DC-activated T cells.

... data by fluorescence microscopy analysing DC distribution in spleen and how this is affected during inflammation driven by stromal cells or hematopoietic cells. One of our images of DC was selected as the cover for the June 2007 issue of Nature Immunology.