Studies of antigen presentation and cross-presentation by DC, inc. electroporation

We obtained data from an evaluation whether antigens could be rerouted from the endosomal into the cytosolic compartment by combining antigen with cell penetrating peptides to further enhance cross-presentation.

We made significant progress using biodegradable polymers to enhance cross-presentation of loaded antigens, and the best results were obtained by maturing DCs with a combination of the TLR ligands R848 and polyI:C. Furthermore, we have shown that DC electroporated with melanoma antigen RNA express the proteins ex vivo and in vivo.

• molecule type
• receptor DC-SIGN,
• resiquimod,
• Major histocompatibility complex class II,
• melanoma antigen,
• Toll like receptor,
• Major histocompatibility complex class I,
• Polyinosinic acid:polycytidylic acid

• cell type
• dendritic cell

• cell component type
• endosome,
• cytosol

• experimental design type
• in vivo design experiment,
• ex vivo design experiment

created over 15 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]