Data has been obtained in a study of antigen recognition of blood-derived T cells. In this study, an IFNgamma ELISPOT was done with PBMC loaded with peptide pools, each consisting of 10 15-mers, which overlap with 11 aminoacids.

...een obtained on the antigen recognition of DTH infiltrating T cells. To obtain the data, activation of CD4 and CD8 T cells upon recognition of EBV-B cells expressing vaccinal antigens was evaluated by CD137 upregulation, CD107a and CD40L expression using flow cytometry. Luminex technology was used t...

Transcriptomic data has been generated on PB-monocytes in healthy controls, RA and SLE.

Data has been obtained on expression and regulations of Notch ligands on DC subsets.

...in the lab of Paola Castagnoli in collaboration with the lab of Juri Rappsilber at IFOM. A pilot project has already been performed on untreated D1 cells and on D1 cells matured for 24 hours with LPS. The two protein sample are currently being analyzed by tandem mass spectrometry by the lab of Jur...

...ed in various conditions. This methodology has been applied successfully to identify: a) a set of proteins specifically associated to phagosomes in DCs but not other phagocytes, such as macrophages, b) a set of proteins specifically recruited to phagosomes during DCs maturation induced by various ...

Data were obtained from rat DC subsets by transcriptional profiling.

We obtained transcriptomic data by analysing the effect of the inhibition of Src family kinases by the specific inhibitors PP1/2 on the uncoupling cytokine production in DCs stimulated by agonists of several Toll like receptors. Whole transcriptome analysis indicated the transcription factor IRF-8 a...

We generated data on the expression by dendritic cells treated with microbial pathogen derivatives.

...ained using Affymetrix mouse chips by designing dendritic cell-based bioassays. To identify possible differentially expressed genes by DC after activation with stimuli typically involved in inducing Th1 or Th2 type responses we have performed comparative kinetic global gene expression analyses of DC...

Gene expression data were generated on DC activated with different TLR agonists typically associated with the respective response.

...e DC-ATLAS effort. They have identified several proteins of interest and are waiting to have a sufficient staffing to characterize their function in DC response to pathogens. Their laboratory is also involved in the pathway curation program initiated by Duccio Cavalieri. They are participating in th...

...ng DC, induced by a prototypical maturation stimulus such as LPS. Results obtained from the LC-MS/MS analysis of the integral plasma membrane proteome of D1 cells stimulated for 24 hours with LPS have been compared to the results obtained from immature D1 cells. As expected, DC up-regulated the cell...

...r required SILAC (stable isotope labelling with amino acids in cell culture) labelling leaving rare cell populations, like in vivo DC subsets, out of reach. In this work package we introduced a robust and generally applicable quantification technology, which allowed us to quantify the dendritic cell...

Our group has generated pathway data from human DC treated with pathogen derivatives.

... and motility of NK cells by tracking migration of DC-primed NK cells in vivo. Since LPS-activated DC are potent inducers of NK cell priming and lymph nodes appear to be a key place in which DC and NK cell interactions occur, we have investigated the in vivo capacity of resting and DC-primed NK cell...

...by mature DCs is critical for long-lasting contacts with CD8+ T cells, but dispensable for short-lived antigen-specific interactions. Serial brief T cell-dendritic cell contacts induced early CD8+ T cell activation, proliferation and effector CTL differentiation in the first few days after immunizat...

... sensing of translation) to monitor translation by FACs in individual or cell populations. We have demonstrated that translation activation is an excellent read-out for early antigen specific T cell activation by DCs. We hope that this technology will become rapidly popular among the DC-THERA partic...

Flow cytometric analysis data were obtained of translation activation in DC-activated T cells.

We have obtained data by fluorescence microscopy analysing DC distribution in spleen and how this is affected during inflammation driven by stromal cells or hematopoietic cells. One of our images of DC was selected as the cover for the June 2007 issue of Nature Immunology.