DCs are washed.

DCs are washed.

The pellets are washed with 70% Ethanol two times.

Hybridized slides are washed in a solution of water, 20× SSC, and 10% SDS, rinsed in water and 20× SSC, and dried via centrifugation for 2 min at 1000 rpm.

DCs are collected and washed 3 times with PBS.