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Protocol Steps  >  adding substance to cell culture step  >  Pulsing with tumor antigen ...

Pulsing with tumor antigen derived peptides

adding substance to cell culture step

Protocol: Generation of monocyte-derived DC under GMP conditions

On day 5 cells are counted and pulsed with tumor antigen derived peptides (one per culture) at a concentration of 5 µg/mL in approximately 5 mL and at a cell density of 20 x 10e6/mL for 1 h.

created over 16 years ago (2 March 2009)    last modified over 13 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]