Pulsing with tumor antigen derived peptides
comment: On day 5 cells are counted and pulsed with tumor antigen derived peptides (one per culture) at a concentration of 5 µg/mL in approximately 5 mL and at a cell density of 20 x 10e6/mL for 1 h.
type: Protocol step
type: adding substance to cell culture step
has biomaterial input: tumor antigen derived peptides
has biomaterial input: MoDC (monocytes after culture with IL-4 and GM-CSF)
has biomaterial output: DC (pulsed with tumor antigen derived peptides)