Pulsing with tumor antigen derived peptides
On day 5 cells are counted and pulsed with tumor antigen derived peptides (one per culture) at a concentration of 5 µg/mL in approximately 5 mL and at a cell density of 20 x 10e6/mL for 1 h.
MoDC (monocytes after culture with IL-4 and GM-CSF)
DC (pulsed with tumor antigen derived peptides)