Protocol for the identification of phagosome proteins in dendritic cells
Laboratory protocolThis methodology incorporates a unique sample preparation procedure based on 96 well plate complex sample trypsinization, a nano-LC-based peptide separation coupled to MSMS analysis sequencing. This procedure led us to the identification of 600 phagosomal proteins from murine DC phagosomes.
Protocol Steps:

created over 16 years ago
(2 March 2009)
last modified over 14 years ago
(28 September 2011)
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