Protocol for the identification of phagosome proteins in dendritic cells
Laboratory protocolThis methodology incorporates a unique sample preparation procedure based on 96 well plate complex sample trypsinization, a nano-LC-based peptide separation coupled to MSMS analysis sequencing. This procedure led us to the identification of 600 phagosomal proteins from murine DC phagosomes.
Protocol Steps:
created over 16 years ago (2 March 2009) last modified over 13 years ago (28 September 2011)  [ RDF ]  [ RelFinder ]