sulfo-NHS-LC-biotin

10 mg/ml sulfo-NHS-LC-biotin (Sigma-Aldrich) in 50 mM NaHCO3 pH 8.5 were used to biotinylate yeasts / spores for 2 hours at 4°C. The remaining reactive biotin molecules were inactivated by incubation in 100 mM Tris-HCl pH 8.0 for 40 minutes at 4°C. DCs were then treated with biotinylated spores/yeasts.

created over 14 years ago (8 February 2010)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]