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Bio Materials  >  molecular structure  >  Molecular entity  >  enzyme  >  sulfo-NHS-LC-biotin

sulfo-NHS-LC-biotin

enzyme

10 mg/ml sulfo-NHS-LC-biotin (Sigma-Aldrich) in 50 mM NaHCO3 pH 8.5 were used to biotinylate yeasts / spores for 2 hours at 4°C. The remaining reactive biotin molecules were inactivated by incubation in 100 mM Tris-HCl pH 8.0 for 40 minutes at 4°C. DCs were then treated with biotinylated spores/yeasts.

created over 13 years ago (8 February 2010)    last modified over 11 years ago (28 September 2011)   [ RDF Rdf ]   [ RelFinder Relfinder ]