Plasmids coding for LACK linked to either eGFP or mCherry fluorescent proteins

We have prepared two plasmids coding for LACK linked to either eGFP or mCherry fluorescent proteins, under the control of the beta-casein promoter.

In order to identify the APCs that were responsible for the development of this state of immune tolerance, we aimed to generate a transgenic mouse that express a recombinant fluorescent protein in the mammary glands, an approach that should result in high levels of expression of the fluorescent protein in the milk. We thus should be able to detect the cells that capture, degrade and present this antigen to T cells. Since we had previously generated a monoclonal antibody, 2C44, that reacts to a peptide derived from the Leishmania LACK protein bound to I-Ad MHC class II molecules, we chose to use LACK as a model antigen in these experiments.

These constructs have been injected into fertilized eggs and transgenic animals are expected for march 2009.

• molecule type
• Leishmania LACK protein

• organ type
• mammary glands

created over 15 years ago (15 December 2009)    last modified over 13 years ago (28 September 2011)   [ RDF ]   [ RelFinder ]