Our laboratory has a FACS Canto II (Manufacturer: BD Biosciences - URL: http://www.bdbiosciences.com/features/products/display_product.php?keyID=594) at its disposal. For instance, we used multimers kindly provided by Pierre Coulie (Brussels) together with surface markers (such as CD8, CD4, CD3, CD45RA, CCR7, CD25, CD27, CD137, CD127) in different 8-color panels on this FACSCanto II.