This flow cytometer was used eg., for an analysis of CD11c populations in the lung and spleen during acute aerosol MTb infection.

There is a GMP facility for conduction of phase III clinical trials available. The structural up-grade of this facility has limited the number of patients recruited.

Genopolis hosts a microarray platform that uses the Affymetrix GeneChip® microarray technology for genome and transcriptome analyses. In 2008, this platform was enhanced by the incorporation of a new institute [the Wellcome Trust Centre for Human Genetics] to provide ‘Illumina’ microarray profiling. The following services are currently available: Whole genome genotyping applications, which include: 1. family-based association studies 2. cancer genetics 3. population genetics 4. chromosomal copy number analysis Whole genome gene expression profiling application...

An Illumina transcriptional profiling platform is available for microarray analyses.

A GenePix 4000 microarray scanner is available in our lab (manufacturer: Axon, Foster City, CA).

The FAX analyser available in the laboratory is used, e.g., to measure transfection efficiency.

The flow cytometer at the disposal of the laboratory is used, for example, to assess Th subsets.

The flow cytofluorometer available to the laboratory is used, for example, for the assessment of differentiation, maturation, and intracellular cytokine production of DCs.

The FACS analyser available to the laboratory is used, for instance, to analyse stained cells.

We have a flow cytometer analyzer at our disposal.

The FACS analyzer avaiable to the lab is used, among other things, to assess protein expression of tumor antigens or soluble proteins.

The gene gun system available to the laboratory was used, e.g., for micro-particle bombardment of murine skin.

This FACS analyser that is available to us is used, e.g., for the analysis of dendritic cells.

We have expertise in DNA vaccination with the gene-gun available to us to directly transfect skin DC in vivo either with expression plasmids or siRNA plasmids to knockdown target genes and follow the effect on the resulting T cell response.

For our work, we have a fluorescence microscope at our disposal. We use it, among other things, to analyse DC distribution in spleen and how this is affected during inflammation driven by stromal cells or hematopoietic cells.

We have used the electron microscope that is available to our lab, e.g., to document the production and secretion of tubulovesicular structures by cells overexpressing VSV-G glycoprotein and for documentation of production and secretion of tubulovesicular structures.

Our laboratory has an electroporator at its disposal. It is used for instance for RNA electroporation into DC.

We use the electroporator at our disposal, for instance, to electroporate dendritic cells with melanoma antigen RNA .

Our laboratory has an electroporator at its disposal. It was used, e.g., for an assessment whether functional activation of human monocyte-derived DCs can be achieved by electroporation of an activation signal in the form of double-stranded (ds) RNA and whether simultaneous electroporation of the dsRNA with tumor antigen encoding mRNA can lead to the induction of a cytotoxic T-lymphocyte (CTL) response.

A Gambro BCT Elutra Cell Separation System is available to us. We have experience, e.g., in using it for monocyte enrichment.