This FACS analyser that is available to us is used, e.g., for the analysis of dendritic cells.

The flow cytofluorometer available to the laboratory is used, for example, for the assessment of differentiation, maturation, and intracellular cytokine production of DCs.

The flow cytometer at the disposal of the laboratory is used, for example, to assess Th subsets.

This flow cytometer was used eg., for an analysis of CD11c populations in the lung and spleen during acute aerosol MTb infection.

We have a flow cytometer analyzer at our disposal.

For our work, we have a fluorescence microscope at our disposal. We use it, among other things, to analyse DC distribution in spleen and how this is affected during inflammation driven by stromal cells or hematopoietic cells.

Genopolis hosts a microarray platform that uses the Affymetrix GeneChip® microarray technology for genome and transcriptome analyses. In 2008, this platform was enhanced by the incorporation of a new institute [the Wellcome Trust Centre for Human Genetics] to provide ‘Illumina’ microarray profiling. The following services are currently available: Whole genome genotyping applications, which include: 1. family-based association studies 2. cancer genetics 3. population genetics 4. chromosomal copy number analysis Whole genome gene expression profiling application...

The gene gun system available to the laboratory was used, e.g., for micro-particle bombardment of murine skin.

We have expertise in DNA vaccination with the gene-gun available to us to directly transfect skin DC in vivo either with expression plasmids or siRNA plasmids to knockdown target genes and follow the effect on the resulting T cell response.

This gene gun was used for the delivery of vectors engineered to express distinct chemokines at the local skin site of mice.

A GenePix 4000 microarray scanner is available in our lab (manufacturer: Axon, Foster City, CA).

There is a GMP facility for conduction of phase III clinical trials available. The structural up-grade of this facility has limited the number of patients recruited.

An Illumina transcriptional profiling platform is available for microarray analyses.

The integral plasma membrane proteome of D1 cells stimulated for 24 hours with LPS has been analyzed with this LC-MS/MS analyser and compared to the results obtained from immature D1 cells.

We have access to and experience with using a linear ion trap / Fourier Transform (LTQ-FT) hybrid mass spectrometer. For example, we use it to analyse purified tryptic peptides.

A comprehensive protein inventory of clinical grade immature and cytokine cocktail matured (Il-6, IL-1 beta, TNF alpha, PGE2; 48 hours) monocyte derived human dendritic cells (DC) from a healthy donor has been established by using high accuracy, high sensitivity protein identification technology. We have identified 2794 proteins in DCs by liquid chromatography tandem mass spectrometry.

A Luminex flow cytometer is available to our lab. It is used, among other things, to quantify the TNFalpha and IFNgamma secretion in a study on antigen recognition.

There is a Luminex flow cytometer available in the lab. It is used, among other things, for assaying of cytokines in B-CLL patients treated with Apo-DC +/- GM-CSF.

There is a MACS separator (Miltenyi Biotech) at the disposal of our lab. It is used, for example, to purify CD8 T cells.

Our laboratory has a mass spectrometer at its disposal.