BD FACS AriaFACSAria
We have a FACS Aria cell sorter at our disposition from BD Biosciences (URL: http://www.bdbiosciences.com/features/products/display_product.php?keyID=53).
We used this FACS Aria among other things for fluorescence activated cell sorting for proteomic investigations. Using Fluorescence activated cell sorting allowed us to obtain highly pure fractions of CD8+, CD4+ and DN DC subsets. However, because DC are a very rare population, this method is very material- and cost-intensive. Furthermore, it is not surprising that the obtained cell numbers of each subset after isolation are below 107. However, these cell numbers should be sufficient for proteomic investigations.