... directing migration of TH17 cells at sites of inflammation or infection. We found that CCR6–deficient (CCR6-KO) mice were resistant to induction of EAE but became susceptible when transferred with small number of CCR6-sufficient T cells. CCR6 was found to be required on a first wave of TH-17 cell...

...h p53 peptides in Montanide adjuvant in patients with colorectal cancer or ovarium cancer. This vaccine was well tolerated and robust p53-specific T cell responses were induced. In the future we intend to combine the p53 vaccine with other cancer therapies such as chemotherapy.

...ined on TriMix DCs co-electroporated with whole tumorantigen encoding mRNA. A critical factor determining the effectiveness of currently used dendritic cell (DC)-based vaccines, is the DC’s activation or maturation status. We have shown recently that the T cell stimulatory capacity of DCs pulsed...

...LR4) encoding mRNA. The effectiveness of the dendritic cell (DC) vaccination protocols that are currently in use could be improved by providing the DCs with a more potent maturation signal. We therefore investigated whether the T-cell stimulatory capacity of human monocyte-derived DCs could be incr...

...ysis of different peptide specific T cell functions like degranulation and production of cytokines such as IFN? and TNF? by 8-color flow cytometry. In general, pre-existing immune-responses were rather mono-functional with dominating “IFN?-only”, “TNF?-only” and “degranulation-only” cell...

...e goal of this initiative is to obtain sufficient genomic data and a reference data set from retrospective studies of a large number of stored human DC samples to understand the genetic basis of inter-donor variability in responses to DC maturation stimuli. ‘Illumina’ microarrays rather than the...